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DNA-based identification of South Korean Megaloptera larvae with taxonomic notes

Published online by Cambridge University Press:  26 June 2015

Sang Woo Jung ,
Tatyana S. Vshivkova  and
Yeon Jae Bae
Sang Woo Jung
Affiliation:
Department of Life Science, Graduate School, Korea University, Seoul 136-713, South Korea
Tatyana S. Vshivkova
Affiliation:
Institute of Biology and Soil Science, Russian Academy of Sciences, Far East Branch, Vladivostok 690022, Russia
Yeon Jae Bae*
Affiliation:
Division of Environmental Science and Ecological Engineering, Korea University, Seoul 136-713, South Korea
*
1Corresponding author (e-mail: yjbae@korea.ac.kr).
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Abstract

Mitochondrial DNA (mtDNA) sequences, which serve as DNA barcodes, have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochrome c oxidase subunit I (COI) gene sequences for South Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we associated five types of larvae with known adults including the following four species of newly described larval stages: Sialis annae Vshivkova, 1979 (Megaloptera: Sialidae); Neochauliodes formosanus (Okamoto, 1910) (Megaloptera: Corydalidae); Parachauliodes asahinai Liu et al., 2008 (Megaloptera: Corydalidae); and Protohermes xanthodes Navás, 1913 (Megaloptera: Corydalidae). The known larval stage of Sialis koreana Jung and Bae, 2012 (Megaloptera: Sialidae) was confirmed, and the morphological variation in the male genitalia of Sialis longidens Klingstedt, 1932 (Megaloptera: Sialidae) is discussed. A larval key to the six South Korean species of Megaloptera is provided.

Type
Systematics & Morphology
Information
The Canadian Entomologist , Volume 148 , Issue 2 , April 2016 , pp. 123 - 139
Copyright
© Entomological Society of Canada 2015 

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Footnotes

Subject editor: Christopher Buddle

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