S56 Abstracts / IBRO Reports 6 (2019) S54–S345 brain’s reward system. It is known that the PFc is the source of a major glutamatergic innervation in the VTA and the NAc, and that cortical control of these brain regions is crucial in reward-related behaviour. However, exact details of these corticofugal pathways are unknown. Therefore, with single retrograde tracing experiments, first, we mapped the prefrontal source of these connections. Our preliminary results indicated that VTA-projecting cells formed two clusters in the PFc – one in the deepest layer and one more superficially. In contrast, NAc-projecting cells were localised in one cluster in the PFc. Then, with double retrograde approach, we investigated the proportion of PFc cells innervating both regions. Finally, to further dissect the anatomy of the cortical innervation of the reward system, we injected adeno-associated viral constructs into the PFc to anterogradely label its connectivity with the mesolimbic system. Our results help to clarify the internal connectivity of the reward system. Our data together opens new opportunities to investigate the cortical control of mesolimbic reward system in a cell-type specific manner. Furthermore, it may help us better understand reward-related processes and their malfunctions (e.g. addiction, depression). https://doi.org/10.1016/j.ibror.2019.07.185 P00.07 Slow-wave sleep are dissociated from paradoxal sleep after contextual fear extinction in rats Luiz Henrique Santana 1,∗ , Karin Moreira 2 , Paula Ayako Tiba 3 1 University of São Paulo, São Paulo, Brazil Federal University of São Paulo, São Paulo, Brazil 3 Federal University of ABC, São Bernardo do Campo, Brazil 2 Increases in paradoxical sleep (PS) after pavlovian extinction sessions was previously described. However, previous works did not test more than one day of extinction and did not test whether sleep modifications after extinction are dependent upon the time interval between conditioning and extinction. We explored sleep architecture modifications on different conditions of conditioned fear extinction learning. We adapted a contextual fear-conditioning task (CFC) in order to explore the effect of an aversive event and a possible effect of time interval between conditioning and extinction session on sleep and behaviour. The first group had one day of CFC training and 5 sessions of extinction starting a week after CFC. The second group, was trained in CFC and exposed to one extinction session a week after CFC. The third group received an immediate shock right after entering the conditioning chamber followed by 5 sessions of re-entering the conditioning chamber. Sleep-wake information was recorded by collecting electrocorticogram and electromyogram data and scored as one between three phases: awake, slow wave sleep (SWS) and PS. Results showed that SWS increased after CFC, and it also showed that PS increased after CFC and extinction for each group. Our findings support previous findings on PS relation with extinction learning and suggest some time-dependent SWS modification for early extinction re-exposure. The discovery of the participation of PS in contextual fear extinction and SWS role on nuances of extinction procedure expands the understanding of behaviour and sleep relations and, at the same time, offer a behavioural model to study sleep dependent stressful memory related to PTSD or HPA axis without the side effects of Electric Shock. https://doi.org/10.1016/j.ibror.2019.07.186 P00.08 Frontal EEG alpha asymmetry changes while watching emotional film clips and role of difference pair of frontal electrodes: A preliminary study Wichulada Suwannapu 1,∗ , Natcharee Kraiwattanapirom 1 , Suradate Prayoonsak 2 , Vorasith Siripornpanich 1,∗ 1 Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand 2 Department of Educational Psychology and Guidance, Mahasarakham University, Mahasarakram, Thailand Frontal alpha asymmetry (FAA) has been found to be a neurobiomarker of affective styles associated with emotional valence and motivational direction. It has been hypothesized the greater left frontal activation reflects the positive (approach) behaviors, whereas the greater right frontal activation reflects the negative (withdrawal) behaviors. However, the role of hemispheric lateralization on emotions is still debated. Moreover, the frontal electrodes consisting of F4/F3 and F8/F7 pairs are commonly used in FAA analysis, but few studies have examined the role of different frontal electrode pairs. This study aimed to clarify the FAA in response emotional clips comparing to resting-state and the influence of difference pairs of frontal sites (F4/F3: medial–frontal sites vs. F8/F7: lateral–frontal sites). The participant were 10 healthy females (aged 20–32 years; M = 25.80, SD = 4.85). Two sets of emotional clips (4 clips per set) were used to elicit target emotional states (sadness, fear, happiness, and neutral). The FAA was calculated using the equation: ln[right] − ln[left] alpha power (8.5–13 Hz). The results found the significantly decreased FAA at F4/F3 pair in response to negative clips (sad; t(9) = 2.787, p = 0.021, fear; t(9) = 2.344, p = 0.044) and significantly increased FAA at F8/F7 pair in response to neutral clips (t(9) = −2.902, p = 0.018) when compared to resting-state. Moreover, the significantly changes among emotional clips can be detected from FAA. All FAA scores from F4/F3 were positive, whereas most FAA scores from F8/F7 were negative. These findings indicated that FAA is associated with emotional response, especially with negative stimuli, and is still valuable tool for studying affective response. However, the selection of frontal electrode pairs is also important. Finally, the difference of alpha power between left and right frontal electrodes seem to be more important than positive or negative FAA values. https://doi.org/10.1016/j.ibror.2019.07.187 P00.09 Chronic activation of 5-HT2A receptors with highly selective agonists affects the behavior and the BDNF system of C57Bl6/J mice Tatiana Ilchibaeva ∗ , Anton Tsybko, Elena Filimonova, Dmitriy Eremin, Nina Popova The Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Serotonin 5-HT2A and brain-derived neurotrophic factor (BDNF) are involved in the pathology or treatment of many psychiatric diseases. However, the role of 5-HT2A receptors in the regulation of the BDNF system in vivo was not examined using highly selective agonists. In present work, the effects of 5-HT2A receptor chronic activation with mixed 5-HT2A/2C receptor