CN111233578A - Composition and method for biologically controlling orobanche coerulescens - Google Patents

Abstract

A composition and a method for biologically controlling orobanche coerulescens, wherein the composition mainly comprises the following components: soil conditioner, microbial agent, soil fertility balancing agent and organic trace element nutrient. The method comprises the following steps: (1) improving saline and alkaline soil: from the first drip irrigation after sowing or transplanting, drip irrigating the soil conditioner into the soil; (2) the microbial bacteria control broomrape: dripping the microbial inoculum into soil from the second drip irrigation after sowing or transplanting; (3) and (3) soil fertility balance adjustment: from the second drip irrigation after sowing or transplanting, drip irrigation the soil fertility balancing agent into the soil; (4) leaf surface nutrition supplement: during the growth period of crops, the organic trace element nutrient is sprayed to the foliage of the crops. The composition can effectively inhibit orobanche coerulescens, can be continuously cultivated, improves the soil salinity and alkalinity, improves the soil fertility, has good benefits of increasing the yield and the income, is environment-friendly, and reduces the using amount of water and fertilizer. The method is scientific, simple and convenient, has low cost and is suitable for agricultural application.

Description

Composition and method for biologically controlling orobanche coerulescens

Technical Field

The invention relates to a composition and a method for controlling orobanche coerulescens, in particular to a composition and a method for biologically controlling orobanche coerulescens.

Background

Broomrape is a generic term for plants of the genus orobanche (orobanche phelipanche) of the family orobantaceae (Orobanchaceae) where roots of parasitic plants live. The orobanche is also called striga asiatica, rhus toxicodendron and Hovenia dulcis, is a parasitic weed which is common in agricultural production and can seriously affect the crop yield, is mainly distributed in Xinjiang, Gansu, Shanxi, inner Mongolia, Heilongjiang, Liaoning, Jilin and other provinces domestically, has wide host plants, is parasitic on Hami melon, watermelon, melon, pea, broad bean, carrot, celery, tobacco, medlar, flax, pepper, tomato, sunflower and other plants to cause harm, and has the advantages of yield reduction and quality reduction in light weight and top dead weight.

Broomrape is a total parasitic weed, all nutrition and moisture are obtained from a host in a root parasitic mode, and due to the special life history of the broomrape, the harm to crops mainly occurs in the underground growth stage after emergence of seedlings, so that the crop production is reduced in all countries in the world in the crop growth process. Herba Orobanches is difficult to prevent and control, and is mainly due to strong ecological adaptability and serious parasitism in saline-alkali soil environment. Each broomrape can produce a large amount of seeds, each broomrape contains 5-100 ten thousand seeds, and each broomrape not only produces a large amount of seeds, but also can maintain the viability for decades in saline-alkali soil. Broomrape growth mainly comprises the following processes: the host root is found by the germination and germination pipe, the suction device is formed and is adhered to the root of the host, the connection with the xylem of the host is successfully established, the nutrition and the water are absorbed from the host to carry out the underground growth, finally, the earth surface is grown out to bloom and produce a large amount of seeds, and a large amount of seed is accumulated in the soil, so that the continuous planting cannot be realized, and only the non-planted soil can be selected for continuous planting. According to investigation, the area of crops (sunflower, melon, processed tomato, watermelon, hot pepper, and the like) damaged by the broomrape plants in Xinjiang is about 100-150 ten thousand acres, the loss caused by agricultural production in China is more than 10 million yuan each year, especially, the planting bases of the sunflower, the tomato and the melon are aggravated year by year, the generation area has a further expansion trend, and the economic development of the industries of the sunflower, the melon, the tomato, and the like is severely restricted.

At present, the conventional methods such as artificial weeding, chemical prevention and removal and the like adopted in the occurrence areas do not have good effects.

The artificial control seems to be capable of removing a certain amount of broomrape, but the defects are obvious, firstly, the broomrape has a short growth period, can continuously germinate, continuously parasitize and continuously grow in the growth process of the tomato, and has no fixed growth season as long as the conditions are proper, and secondly, the broomrape mainly absorbs nutrients and water in the body through connecting roots of the crops, and the roots of the crops are inevitably injured when the broomrape is removed, so that the growth of the crops is influenced. The traditional Chinese medicine is eradicated by deep ploughing and weeding, but is time-consuming and labor-consuming and has poor effect.

The chemical control agent generally has no broad spectrum for broomrape, only withers and dies the soil above the broomrape, but has no effect on stems below the soil surface and broomrape seedlings.

And also kills broomrape seeds by an effective agent.

CN105028486A discloses a method for preventing and controlling weed broomrape by matching agricultural biodegradable mulching film with biocontrol bacteria, which comprises obtaining fermentation liquor of pythium aphanidermatum; centrifuging the obtained fermentation liquor, eluting the fermentation liquor on a column, and concentrating to obtain pythium aphanidermatum powder; preparing a biodegradable mulching film containing the pythium aphanidermatum powder; the mulching film is paved in the field with crops and broomrape. However, the method needs to dissolve the medicament and the biodegradable mulching film, has great operation difficulty and difficult realization, has certain limitation in agricultural production operation, and particularly greatly increases the production cost when being applied in a large area.

CN106961985A discloses a tomato planting method capable of preventing parasitic tomatoes on orobanche coerulescens, which is characterized in that two microbial inoculants are prepared by adopting inorganic components of urea, ferrous sulfate and a cooked cow dung extracting solution, corn hosts are planted in a combined manner, orobans seeds are induced, and corns are harvested in advance when the orobans seeds are immature, so that the parasitic of part of orobans coerulescens seeds in soil can be solved. However, a large amount of seeds still exist in the soil, and continuous cropping planting is difficult to realize.

CN107041358A discloses a method for controlling parasitic plant orobanche coerulescens, a special preparation thereof and application of the preparation, wherein the key point is that the monolaurone biosynthesis inhibitor TIS108 is utilized to prepare the special preparation to treat the orobanche coerulescens seeds, so as to promote the seeds to germinate in the non-growing period of crops, and the seeds die because no host can be found after germination, thereby achieving the purpose of effectively reducing the orobanche coerulescens seed bank in soil. However, in agricultural production, since the spread of the broomrape seeds in the soil cultivation layer is wide, the induction of the soil broomrape seeds in its entirety has certain limitations, and particularly, the application in a large area greatly increases the production cost.

CN109729778A discloses the use of 5-aminolevulinic acid for increasing resistance of sunflower to the parasitic weed, sunflower broomrape, and further finds that 5-aminolevulinic acid increases resistance to sunflower broomrape by promoting antioxidant enzyme synthesis in sunflower plants, reducing levels of MDA and ROS in sunflower plants, and increasing GSH concentration and GSH/GSSG ratio in sunflower plants; effectively relieving the problem that the sunflower broomrape parasitizes to influence the growth of the sunflower in the planting process of the sunflower. However, the technical production cost of 5-aminolevulinic acid is high, and each kilogram of the 5-aminolevulinic acid is 2-3 ten thousand yuan, so that the production cost is greatly increased if the 5-aminolevulinic acid is applied to agricultural production.

Mayongqing et al propose a method for controlling by trapping crops and trapping crops, which is a method for trapping and controlling the broomrape weeds by stimulating the germination of seeds with a Chinese herbal medicine preparation. However, because the method adopts the mode of trapping the soil broomrape seeds which are widely distributed, the damage of the broomrape to the crops in the same season cannot be eliminated in the actual planting process, the large-area popularization and application are difficult to realize, and the continuous planting (Mayongqing and the like. broomrape weeds and the prospect of prevention and control measures. China biological control reports [ J ],2012,28(1): 133-.

In addition, Xujiao et al utilize the toxin produced by pythium aphanidermatum to perform Takara Shuzo control, however, the control efficiency of pythium aphanidermatum in this method is limited and the duration of the effect is also limited by the mode of application (Xujiao et al. isolation and identification of the herbicidal active ingredient of pythium aphanidermatum. China agricultural science [ J ],2009,42(6): 1994-2001). The broomrape also uses directly synthesized chemical effective substances for controlling, but the chemical pesticide has short half-life period, short retention time of effective components in soil, short pesticide effect period and use defect.

Zeng Weidong and the like disclose a prevention effect and yield increase test of the Shenhoe composite microbial fertilizer on tomato broomrape, which is a demonstration that tomato broomrape is prevented and controlled by adopting a single microbial bacterium method in northern great canal village of Yanqi county in Xinjiang in 2013, and the prevention and control rate of broomrape in the same year can reach 90% (Zeng Weidong and the like, a prevention effect and yield increase test of the Shenhoe composite microbial fertilizer on tomato broomrape [ J ] modern agricultural science and technology, 2016,673(11): 97). Zengweidong and the like disclose a compound microbial fertilizer for soil conditioner and Shenhoe to prevent and treat the synergistic effect of tomato broomrape, which is a demonstration that a soil conditioner and a microbial fertilizer are combined to prevent and treat the tomato broomrape, and the effect of preventing and treating the broomrape can reach 84.3 percent (Zengweidong and the like, the compound microbial fertilizer for soil conditioner and Shenhoe to prevent and treat the synergistic effect of tomato broomrape [ J ] rural science and technology, 2018(4): 31-33.). However, both of the above methods have the same technical drawbacks: 10% of the broomrape seeds still exist in the soil without germination, as long as one broomrape seed is parasitic in the soil in the second season, tens of thousands or hundreds of thousands of broomrape seeds exist, and in the subsequent planting process, the broomrape seeds are parasitic to cause harm, so that the method cannot radically solve the harm of the broomrape.

Cinnamel and the like disclose a comprehensive experimental technical research on the biological control of broomrape, which utilizes a microbial agent to control the broomrape. Although the control effect can reach 63 percent, the control effect of increasing yield and controlling sunflower broomrape is certain, in terms of control efficiency, more than 30 percent of broomrape seeds still exist in soil, and the sunflower seeds cannot be continuously planted in the same land (Zhu Ming et al.

Plum Chi Qiang and the like disclose a field test for preventing and controlling flue-cured tobacco broomrape by a Shenhoe compound microbial fertilizer, which utilizes a microbial agent to prevent and control the tobacco broomrape. Although the control effect can reach 67 percent, the control effect has certain effects of increasing the yield and controlling tobacco broomrape, more than 30 percent of broomrape seeds still exist in the soil in terms of control efficiency, and the tobacco can not be continuously planted in the situation (Lizhiqiang et al. "Shenhoe" compound microbial fertilizer control flue-cured tobacco broomrape field test [ J ]. New agriculture, 2016, 7 th period: 58-59.).

The existing scheme of crop rotation and stubble rotation advocates that crop rotation and stubble rotation can be carried out for 4-6 years or even longer in continuous cropping and multi-stubble fields, but for the survival time of broomrape seeds in the field being more than 10 years, the scheme can not solve the broomrape hazards in continuous planting, the effect or economic benefit of stubble cleaning and weeding is poor, and the land utilization rate is not suitable for the sustainability of local industries.

In summary, a composition and a method for biologically controlling the herba orostachyos, which are scientific, simple, efficient, capable of inhibiting herba orostachyos, capable of continuously cultivating and planting, improving the soil salinity and alkalinity, improving the soil fertility, increasing the yield and income, good in benefit, environment-friendly, low in cost and suitable for agricultural application, are urgently needed to be found.

Disclosure of Invention

The invention aims to solve the technical problem of overcoming the defects in the prior art and provide a biological control herba Orobanches composition which has the advantages of high efficiency in inhibiting herba Orobanches, continuous cultivation, improvement of soil salinity and alkalinity, fertility improvement, good yield increase and income increase benefits, environmental friendliness and reduction of water and fertilizer consumption.

The invention further aims to solve the technical problem of overcoming the defects in the prior art and provide a method for biologically controlling the broomrape, which is scientific, simple, convenient, low in cost and suitable for agricultural application.

The technical scheme adopted by the invention for solving the technical problems is as follows: a composition for biologically controlling orobanche coerulescens mainly comprises the following components: soil conditioner, microbial agent, soil fertility balancing agent and organic trace element nutrient. The soil conditioner can improve the salinity and alkalinity of the soil of the planted crops. The microbial agent can generate organic acid and biological enzyme, the biological enzyme can inhibit the germination of the broomrape seeds and kill the broomrape seeds in the soil, and meanwhile, the microorganism can improve the utilization rate of the fertilizer, improve the organic matters of the soil, improve the fertility of the soil, promote the growth of crops and improve the yield, thereby realizing the biological improvement of the soil. According to the nutrient balance principle, the trace element nutrient is applied to the leaf surfaces, so that the leaf surfaces and the stalks of crops can absorb nutrients, the photosynthesis is improved, the wind resistance and drought resistance of the crops are enhanced, and the nutrient is complemented with the soil fertility balancer.

Preferably, the biological control broomrape composition comprises the following components in parts by weight: 1-30 parts (more preferably 16-25 parts) of a soil conditioner, 2-30 parts (more preferably 5-25 parts) of a microbial agent, 80-150 parts (more preferably 85-130 parts) of a soil fertility balancing agent and 0.15-0.60 part of an organic trace element nutrient.

Preferably, the soil conditioner comprises the following main components in percentage by weight: 110-180 g/L of amino acid, 210-280 g/L of fulvic acid and pH value of 2.5-4.5 (more preferably 3.0-4.3). The amino acid in the soil conditioner is amphoteric electrolyte, so that the pH value of the soil can be adjusted, and the pH value of the soil is reduced; amino acid is absorbed and utilized by crops as a nutrient component, and simultaneously can be used as food of a microbial agent to provide nutrition for soil microorganisms, the microorganisms in the soil propagate in a large quantity to improve the soil environment, and the microorganisms provide soil organic matters through metabolism, so that the purpose of fertilizing the soil fertility is achieved; the increase of beneficial flora can also inhibit the propagation of harmful germs in the soil, so as to achieve the purpose of purifying the soil, the healthy soil is more beneficial to the planting and continuous cropping of crops, and the soil can be continuously utilized.

Preferably, the microbial agent is mainly prepared by mixing the microbial agent and a carrier in a mass ratio of 1: 1-70 (more preferably 1: 2-20).

Preferably, the viable count of the microbial agent is 1.0 × 10⁸～1.0×10¹⁰Per gram.

Preferably, the microbial preparation is one or more of a trichoderma viride preparation, a bacillus licheniformis preparation, a bacillus subtilis preparation, a saccharomyces cerevisiae preparation, a bacillus amyloliquefaciens preparation, a bacillus brevis laterosporus preparation, a bacillus mucilaginosus preparation or a pseudomonas palustris preparation and the like. More preferably, the microbial preparation is a mixed bacterial preparation with the mass ratio of the bacillus licheniformis preparation to the bacillus amyloliquefaciens preparation of 1: 0.5-2.0, the mixed bacterial preparation with the mass ratio of the bacillus licheniformis preparation to the bacillus amyloliquefaciens preparation of 1: 0.5-3.0, the mixed bacterial preparation with the mass ratio of the saccharomyces cerevisiae preparation to the bacillus amyloliquefaciens preparation to the trichoderma viride preparation of 1: 0.2-1.0, the mixed bacterial preparation with the mass ratio of the bacillus licheniformis preparation to the saccharomyces cerevisiae preparation to the pseudomonas palustris preparation to the bacillus mucilaginosus preparation of 1: 0.5-2.0: 0.5-3.0, and the mixed bacterial preparation with the mass ratio of the bacillus amyloliquefaciens preparation to the bacillus mucilaginosus preparation of 1: 0.5-3.0. The microbial agent can generate organic acid and biological enzyme (cellulase, protease and the like), the biological enzyme can inhibit the germination of the broomrape seeds, parasitic broomrape roots can decay under the action of the enzyme, the broomrape cannot absorb nutrients, the broomrape growing out of soil dies, broomrape stalks decay into organic materials, and organic matters are supplemented to the soil; after continuous planting, soil microbial enzymes can continuously kill the orobanche coerulescens seeds which are not killed in the first season in the soil, and finally the harm of the orobanche minor to crops is solved, so that the aim of preventing and treating the orobanche minor is fulfilled. Meanwhile, the nitrogen fixation, phosphate dissolution and potassium dissolution functions of the microorganisms can improve the utilization rate of the fertilizer, the metabolism of the microorganisms can improve the organic matters of the soil, improve the fertility of the soil, promote the growth of crops and improve the yield, and achieve the double-effect functions of soil conditioning and biological improvement.

Preferably, the carrier is one or more of attapulgite, bentonite, potash feldspar, amino acid, fulvic acid, humic acid or alginic acid.

Preferably, the soil fertility balancing agent mainly comprises a nitrogen-phosphorus-potassium fertilizer, a secondary element fertilizer and a trace element fertilizer.

Preferably, the soil fertility balancing agent comprises the following fertilizers in parts by weight: 75-120 parts of nitrogen-phosphorus-potassium fertilizer (more preferably 80-100 parts), 4-20 parts of medium element fertilizer (more preferably 5-15 parts), and 1-10 parts of trace element fertilizer (more preferably 2-8 parts).

Preferably, in the nitrogen-phosphorus-potassium fertilizer, the nitrogen fertilizer, the phosphorus fertilizer and the potassium fertilizer are in parts by weight: 1-30 parts of nitrogenous fertilizer, 1-40 parts of phosphate fertilizer and 1-40 parts of potash fertilizer. The specific dosage of the nitrogen fertilizer, the phosphorus fertilizer and the potassium fertilizer can be calculated according to the fertilizer requirement rule of the crop in the growth period.

Preferably, the nitrogen fertilizer is one or more of urea, ammonium sulfate, ammonium nitrate or ammonium chloride.

Preferably, the phosphate fertilizer is one or more of ammonium dihydrogen phosphate, ammonium phosphate nitrate, urea phosphate and the like.

Preferably, the potash fertilizer is one or more of potassium sulfate, potassium chloride, potassium nitrate or potassium dihydrogen phosphate.

Preferably, in the medium element fertilizer, the medium element fertilizer and the weight parts thereof are respectively as follows: 1-20 parts of calcium fertilizer, 1-30 parts of magnesium fertilizer and 1-10 parts of silicon fertilizer.

Preferably, the calcium fertilizer is one or more of calcium nitrate, calcium chloride, calcium sulfate or organic acid chelated calcium and the like.

Preferably, the magnesium fertilizer is one or more of magnesium nitrate, magnesium chloride, magnesium sulfate or organic acid chelated magnesium.

Preferably, the silicon fertilizer is one or more of sodium silicate, potassium silicate or organic silicon.

Preferably, in the trace element fertilizer, the trace element fertilizer comprises the following components in parts by weight: 0.5-4.0 parts of zinc fertilizer, 0.1-1.0 part of manganese fertilizer, 0.1-0.4 part of boron fertilizer, 0.1-0.4 part of copper fertilizer, 0.5-8.0 parts of iron fertilizer, 0.01-0.30 part of molybdenum fertilizer, 0.04-0.40 part of rare earth fertilizer, 0.01-0.05 part of cobalt fertilizer and 0.01-0.20 part of selenium fertilizer. The rare earth fertilizer is soluble salt of one or more of 17 metal elements including lanthanide elements, scandium and yttrium.

Preferably, the zinc fertilizer is one or more of zinc sulfate, zinc nitrate, zinc chloride or organic acid chelated zinc and the like.

Preferably, the manganese fertilizer is one or more of manganese sulfate, manganese nitrate, manganese chloride or organic acid chelated manganese and the like.

Preferably, the boric fertilizer is borax and/or boric acid and the like.

Preferably, the copper fertilizer is one or more of copper sulfate, copper nitrate, copper chloride or organic acid chelated copper.

Preferably, the iron fertilizer is one or more of ferrous sulfate, ferric nitrate, ferric chloride or organic acid chelated iron.

Preferably, the molybdenum fertilizer is ammonium molybdate and/or sodium molybdate and the like.

Preferably, the cobalt fertilizer is cobalt sulfate and the like.

Preferably, the selenium fertilizer is sodium selenite and the like.

Preferably, the organic acid is one or more of ethylenediamine tetraacetic acid, amino acid or citric acid and the like.

Preferably, the organic trace element nutrient comprises the following main components in percentage by weight: 130-300 g/L amino acid, 1-9 g/L calcium, 1-9 g/L magnesium, 0.02-9 g/L zinc (more preferably 0.2-2.0 g/L), 0.02-9 g/L manganese (more preferably 0.2-2.0 g/L), 0.005-5 g/L boron (more preferably 0.01-1 g/L), 0.005-9 g/L copper (more preferably 0.01-0.50 g/L), 0.02-9 g/L iron (more preferably 0.1-5.0 g/L), 0.001-1 g/L molybdenum (more preferably 0.01-0.50 g/L), and 0.001-0.1 g/L selenium (more preferably 0.01-0.08 g/L).

The amino acid used in the present invention is a mixture of isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine, glutamic acid and proline.

The technical scheme adopted for further solving the technical problems is as follows: a method for biologically controlling Oroban, comprising the steps of:

(1) improving saline and alkaline soil: dripping a soil conditioner into soil from the first drip irrigation after sowing or transplanting to improve the saline and alkaline of the soil;

(2) the microbial bacteria control broomrape: dripping the microbial agent into soil from the second drip irrigation after sowing or transplanting, and controlling broomrape by the biological enzyme generated by the microbial agent;

(3) and (3) soil fertility balance adjustment: from the second drip irrigation after sowing or transplanting, drip irrigation the soil fertility balancing agent into the soil for soil fertility balance adjustment;

(4) leaf surface nutrition supplement: and during the growth period of the crops, the organic trace element nutrient is sprayed to the leaf surfaces of the crops to supplement the nutrition of the leaf surfaces.

Preferably, in the step (1), the crop is one or more of watermelon, hami melon, hot pepper, melon, sunflower, watermelon, pea, broad bean, carrot, celery, tobacco, flax or tomato, etc.

Preferably, in the step (1), the total dosage of the soil conditioner is 1-30 kg/mu (more preferably 16-25 kg/mu).

Preferably, in the step (1), 1-7 kg/mu of soil conditioner is subjected to drip irrigation for the first time, 10-50 days are separated in each drip irrigation, and the drip irrigation is carried out for more than or equal to 2 times (more preferably 2-5 times). The soil conditioner has better using effect in the previous period, and the specific interval time is determined according to the condition of the water content of the soil in the growth period of the crops.

Preferably, in the step (2), the total dosage of the microbial inoculum is 2-30 kg/mu (more preferably 5-25 kg/mu).

Preferably, in the step (2), 1-3 kg/mu of the microbial agent is subjected to drip irrigation for the first time, the interval between each drip irrigation is 6-21 days, and the drip irrigation is carried out for more than or equal to 3 times (more preferably 3-6 times) in total. The reason why the microbial agent is used at the beginning of the second drip irrigation is that the ground temperature is low in the early stage, the ground temperature is increased at the beginning of the second drip irrigation, the growth effect of the microorganisms is better after the microbial agent is applied, and the specific interval time is determined according to the condition of the soil water content in the growth period of the crops.

Preferably, in the step (3), the total dosage of the soil fertility balancing agent is 80-150 kg/mu (more preferably 85-130 kg/mu), wherein the nitrogen, phosphorus and potassium fertilizers are 75-120 kg/mu (more preferably 80-100 kg/mu), the secondary element fertilizers are 4-20 kg/mu (more preferably 5-15 kg/mu), and the trace element fertilizers are 1-10 kg/mu (more preferably 2-8 kg/mu).

Preferably, in the step (3), 2-15 kg/mu of nitrogen, phosphorus and potassium fertilizers, 1-3 kg/mu of secondary element fertilizers and 0.5-2.0 kg/mu of trace element fertilizers are subjected to drip irrigation for the first time, wherein the drip irrigation interval is 6-21 days each time, and the drip irrigation is carried out for more than or equal to 3 times (more preferably 3-6 times) in total. The soil fertility balancing agent is used from the second drip irrigation, the dosage is determined according to the growth period of crop varieties planted in the season, a soil testing formula and the like, and the specific interval time is determined according to the condition of the soil water content in the growth period of the crops.

Preferably, in the step (4), the total dosage of the organic trace element nutrient is 0.15-0.60 kg/mu. When the organic trace element nutrient is used, the organic trace element nutrient is diluted by 200-400 times and sprayed on the leaf surface.

Preferably, in the step (4), the organic trace element nutrient is sprayed on the leaf surfaces for 1-3 times, and the spraying interval is 10-35 days every time. Because the salt and alkali in the soil harm the nutrient absorption of the root system, particularly, the trace elements have natural obstacles, and the leaf surfaces supplement nutrient nutrients for the growth of crops, the healthy growth of the crops is promoted, and the high quality and the high yield are realized.

Preferably, in the steps (1) to (4), when the soil conditioner, the microbial agent and the soil fertility balancing agent are applied in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied, and the application interval is 30-60 min. The application is carried out at intervals of 30-60 min, so that the harm to the microbial bacteria caused by the direct contact of the microbial agent and inorganic salt fertilizers such as nitrogen, phosphorus and potassium, secondary elements and trace elements can be avoided.

Preferably, after the soil fertility balancing agent is subjected to drip irrigation, clear water is subjected to drip irrigation for 1-2 hours.

The invention has the following beneficial effects:

(1) the composition for biologically controlling the broomrape can efficiently inhibit the broomrape, can realize continuous cultivation, has a broomrape controlling effect as high as 99.3 percent, has stable effect, and solves the problem that continuous cropping and continuous cropping cannot be realized;

(2) the composition for biologically controlling the orobanche coerulescens can improve the salinity and alkalinity of soil, is easy to biodegrade, is not easy to cause soil hardening and environmental pollution while improving the physical and chemical properties of the soil, can be used for fertilizing the soil and promoting the growth of crops, has the yield increasing rate of 74.4 percent and the input-output ratio of 1:7, does not contain chemical pesticide and herbicide components, is non-toxic and harmless to the crops, is friendly to the soil environment, ensures the health of the soil and the safety of food, reduces the using amount of fertilizer and saves water resources;

(3) in the growth process of crops, the method carries out biological control and Roche by using the bio-organic soil improvement and water-fertilizer integrated drip irrigation technology, utilizes beneficial organisms in an agricultural ecosystem to naturally control pests, is scientific, simple and convenient, is not influenced by geological conditions and soil environment, saves labor and time, realizes continuous planting of crops in the same field, solves the worldwide problem of continuous planting without stubble reversing, and is suitable for agricultural application.

Detailed Description

The present invention will be further described with reference to the following examples.

The amino acid used in the embodiment of the invention is a mixture of isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine, glutamic acid and proline, and is purchased from Longteng Biotech limited, Emblica; the bacillus licheniformis preparation, the saccharomyces cerevisiae preparation, the bacillus amyloliquefaciens preparation, the rhodopseudomonas palustris preparation, the trichoderma viride preparation and the bacillus mucilaginosus preparation used in the embodiment of the invention are purchased from China agricultural microorganism strain preservation management center and Beijing aerospace Hengfeng science and technology, Inc.; the rare earth fertilizer used in the embodiment of the invention is an equal ratio mixture of lanthanum nitrate, cerium nitrate, praseodymium nitrate, neodymium nitrate, promethium nitrate, samarium nitrate, europium nitrate and gadolinium nitrate, which is purchased from Ganzhou rare earth mining company Limited; the nitrogen, phosphorus and potassium fertilizers, the medium element fertilizers and the trace element fertilizers used in the embodiment of the invention are purchased from local agricultural service companies; the organic trace element nutrient used in the embodiment of the invention is purchased from Longteng Biotech limited of Emei mountain; the raw materials, fertilizers, etc. used in the examples and comparative examples of the present invention were obtained from conventional commercial sources unless otherwise specified.

The method of the embodiment of the invention is repeated for 3 times, and the area of each cell is controlled by 20 mu according to the drip irrigation ball valve.

Embodiments 1-5 of a composition for biological control of orobanche coerulescens

The components, parts by weight and contents of the composition for biologically controlling orobanche coerulescens in examples 1 to 5 are shown in tables 1 to 5.

TABLE 1 composition for biological control of orobanche coerulescens compositions examples 1 to 5, the components and parts by weight of which are listed in the table

TABLE 2 composition for biological control of Orobanchum, compositions of soil conditioner in examples 1-5 and content table

TABLE 3 composition for biological control of orobanche coerulescens compositions Components and parts by weight of microbial Agents in examples 1-5

Note: in the table, "-" indicates no addition.

TABLE 4 composition for biological control of Orobanchum broomrape compositions examples 1-5 compositions of soil fertility balancing agent and parts by weight

Note: in the table, "-" indicates no addition.

TABLE 5 composition for biological control of orobanche coerulescens compositions examples 1-5 Components and content tables of organic Trace element Nutrients

Method for biologically controlling orobanche coerulescens example 1

Basic conditions of the test foundation: the test field is arranged in 163 mass 4 continuous cropping melon seed field of Jiu Shi nong, Tacheng, Xinjiang, 700 mu, the previous crop is melon, the occurrence of Lianbang in 2010 is serious, and the yield of the melon seeds in the current year is 116 kg/mu; 45mg/kg of alkaline hydrolysis nitrogen, 15mg/kg of quick-acting phosphorus and 98mg/kg of quick-acting potassium; the test time is 4-9 months in 2011.

And (3) trial article species: the watermelon (New melon seed I) is provided by local agricultural resources through a marketing part.

(1) Improving saline and alkaline soil: according to the components, the weight parts and the contents of the raw materials in the examples 1 and 2 in the tables 1 and 2, when seedling emergence water is firstly dripped in 5 months and 1 day after seeding of the watermelon, the soil conditioner is firstly dripped into the soil at 2 kg/mu, the seedling is sown in the squat and root extending period of the watermelon at 6 months and 10 days, and the soil conditioner is secondly dripped into the soil at 4 kg/mu for soil saline-alkali improvement;

(2) the microbial bacteria control broomrape: according to the components, the weight parts and the contents of the raw materials in the embodiment 1 shown in the tables 1 and 3, when drip irrigation is carried out for the second time in the squat seedling root extension period of the watermelon in 10 days at 6 months after seeding of the watermelon, the microbial inoculum is firstly dripped into the soil at 2 kg/mu, the watermelon is subjected to bud development and flowering period in 1-5 days at 7 months, the microbial inoculum is dripped into the soil at 2 kg/mu for the second time, the seeding grain is dripped into the soil at 20 days at 7 months, the microbial inoculum is dripped into the soil at 2 kg/mu for the third time, the watermelon is subjected to mature period at 1 day at 8 months, the microbial inoculum is dripped into the soil at 2 kg/mu for the fourth time, and the microbial inoculum is used for enzyme control;

(3) and (3) soil fertility balance adjustment: according to the raw material components and weight parts of the components in the examples 1 and 4, when drip irrigation is carried out for the second time in the squat and root extending period of the watermelon 10-15 days after seeding of the watermelon, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into soil at 15 kg/mu, 1.5 kg/mu and 1 kg/mu for the first time, the watermelon is sowed at 1-5 days in 7 months at the bud developing and flowering period, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into soil at 30 kg/mu, 1.0 kg/mu and 0.5 kg/mu for the second time, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into soil at 25 kg/mu, 1.5 kg/mu and 0.3 kg/mu for the third time in the seed filling period of the watermelon 20 days in 7 months, the mature period of the watermelon is sowed at 8 months and the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped at 30 kg/mu, Dripping 1 kg/mu and 0.2 kg/mu into soil for soil fertility balance adjustment;

in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 45 min; after drip irrigation with the soil fertility balancing agent, drip irrigation for 1.5h with clear water;

(4) leaf surface nutrition supplement: according to the components, the weight parts and the contents of the raw materials in the examples 1 and 5 in the table 1, in the growth period of the watermelon, in the squatting seedling root extension period of the watermelon in 10 days in 6 months, the organic microelement nutrient is diluted by adding water by 300 times at 0.1 kg/mu for the first time and is sprayed to the leaf surface of the crop, in the bud development period of the watermelon in 1 day in 7 months, in the flowering period of the watermelon, the organic microelement nutrient is diluted by adding water by 300 times at 0.2 kg/mu for the second time and is sprayed to the leaf surface of the crop, in the grain filling period of the watermelon in 20 days in 7 months, the organic microelement nutrient is diluted by adding water by 300 times at 0.2 kg/mu for the third time and is sprayed to the leaf surface of the crop for the nutrition.

When the Lianbang is biologically prevented and treated, the usual operations of preventing diseases and preventing insects are carried out on the planting of the seed watermelon (New melon seed I).

Comparative example 1

And (4) planting the new melon seeds I, performing conventional fertilization operation, and draining water to emerge.

Comparative example 2

Dripping 2 kg/mu of first water and 4 kg/mu of second water into the soil conditioner of the embodiment 1; and planting the new melon seeds I, and performing conventional fertilization operation.

Comparative example 3

The microbial inoculum of the embodiment 1 is dripped into 2 kg/mu of the second water, 2 kg/mu of the third water, 2 kg/mu of the fourth water and 2 kg/mu of the fifth water along with the fertilizer water, and the total amount is 8 kg/mu; and planting the new melon seeds I, and performing conventional fertilization operation.

Soil is taken before each drip irrigation to test the pH value, total salt and soil organic matter of the soil, and soil change and agronomic characters are observed and recorded as shown in tables 6 and 7.

TABLE 6 change table of soil parameters before and after planting of the watermelon in example 1 and comparative examples 1-3

TABLE 7 comparison table of yield and broomrape incidence of watermelon in example 1 and comparative examples 1-3

As can be seen from tables 6 and 7:

1) the soil conditioner, the microbial agent, the soil fertility balancing agent and the organic trace element nutrient are used in a matching manner, so that the salt can be effectively reduced, the alkali can be removed, the current situation of soil hardening can be improved, the ecological environment of soil can be improved, the permeability of the soil can be enhanced, the formation of a soil granular structure can be promoted, the soil activity can be excited, the soil fertility can be improved, and the soil can be effectively improved and repaired;

2) the yield of the melon seeds is increased by 61.7 percent, the empty shells and blighted grains are reduced, and the seeds are full;

3) the control effect of broomrape is as follows: microbial enzyme production inhibits broomrape of watermelon (new melon seed number one), in the process of 5 times from emergence to harvest of drip irrigation in example 1, observation is recorded for 1 time before each drip irrigation, the first 3 times of investigation of the example of the invention demonstrate that no broomrape appears, the control area of comparative example 1 has 20%, the fourth time of investigation after the drip irrigation demonstrates that the example of the invention demonstrates that there is 1% and only 1.5% after the fifth water, and the broomrape that has occurred stops growing, and the fleshy root that is fat is found to start to turn black and rot, the broomrape that is a little bit smaller begins to die, the broomrape stops growing completely, withers and dies, and the incidence rate of the plot of comparative example 1 is 100%; the effect of inhibiting the broomrape of the watermelon (new melon seed I) in the embodiment of the invention reaches 98.5%.

In conclusion, the composition provided by the embodiment of the invention can improve the soil salinity and alkalinity, improve the yield and reduce the fertilizer usage amount while controlling the orobanche, and is applied by adopting a drip irrigation technology, and the drip irrigation water is only 350-400 m per season³Per mu, the water consumption of the conventional irrigation is 800 m per season³Compared with conventional watering and irrigation, the water consumption is saved by 50 percent per mu, the maximum benefit of water resources is exerted, the labor intensity is reduced by directly adding fertilizer into the fertilizer application tank, and the cost is saved and the efficiency is increased.

Example 2

Basic conditions of the test foundation: the test land is arranged in the muskmelon field of the improved variety field in Fuhai county for 20 mu, the control area is set for 20 mu, the test land is flat, the shape is neat, the fertility is high, and the chestnut calcium soil is used; the method is characterized in that melons are planted in 4 years continuously before, particularly, Ronggan occurs in 2012, the roots of Hami melons are lifted out of the soil surface by dozens of Ronggan, the yield is greatly reduced, and only crop rotation is adopted, wherein the crop rotation mode is watermelon-gourd-watermelon-melon; the test time is 2013, 4-9 months.

And (3) trial article species: hami melon 86-1, provided by local agricultural resources via marketing part.

(1) Improving saline and alkaline soil: according to the components, the weight parts and the contents of the raw materials in the examples 2 shown in the tables 1 and 2, when seedling emergence water is subjected to drip irrigation for the first time in 5-month and 1-day days after the seeding of the Hami melons, the soil conditioner is dripped into the soil at 3 kg/mu for the first time, the squatting seedling of the Hami melons extends to the root in 6-month and 10-day days, the soil conditioner is dripped into the soil at 3 kg/mu for the second time, the Hami melons show buds in 7-month and 1-day periods, and the soil conditioner is dripped into the soil at 4 kg/mu for the third time, so that the saline-alkali improvement of the soil is;

(2) the microbial bacteria control broomrape: according to the raw material components and the weight parts and contents in the examples 2 in the tables 1 and 3, when drip irrigation is carried out for the second time in the 6-month and 10-day squat seedling root extension period of the Hami melons after the Hami melons are sown, the microbial inoculum is firstly dripped into the soil at 3 kg/mu, the Hami melons are firstly irrigated into the soil at the 7-month and 1-day bud development period, the microbial inoculum is secondly irrigated into the soil at 3 kg/mu, the Hami melons are secondly irrigated into the soil at 7-month and 10-day flowering period, the microbial inoculum is thirdly irrigated into the soil at 3 kg/mu, the Hami melons are secondly irrigated into the soil at 7-month and 25-day expansion period, the microbial inoculum is fourthly irrigated into the soil at 2 kg/mu, the Hami melons generate at 8-month and 1-day mature period, the microbial inoculum is dripped into the;

(3) soil fertility balancing agent: according to the raw material components and weight parts of the components in the examples 2 in the tables 1 and 4, when drip irrigation is carried out for the second time in the squat and root extension period of the Hami melon at 10 days in 6 months after the Hami melon is sown, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 15 kg/mu, 2.5 kg/mu and 0.5 kg/mu for the first time, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 20 kg/mu, 2.5 kg/mu and 0.2 kg/mu in the bud development period of the Hami melon at 1 day in 7 months, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 15 kg/mu, 3 kg/mu and 0.4 kg/mu for the second time, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 20 kg/mu, 3 kg/mu and 0.4 kg/mu in the bloom period of the Hami melon at 25, 2 kg/mu and 0.3 kg/mu are dripped into soil, and in the mature period of the Hami melon of 1 day at 8 months, the nitrogen, phosphorus and potassium fertilizers, the secondary element fertilizers and the trace element fertilizers are respectively dripped into the soil at 20 kg/mu, 2 kg/mu and 0.1 kg/mu for the fifth time, so as to carry out soil fertility balance adjustment;

in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 45 min; after drip irrigation with the soil fertility balancing agent, drip irrigation for 1.5h with clear water;

(4) leaf surface nutrition supplement: according to the raw material components and the weight parts and contents of the raw material components in the table 1 and 5, in the growth period of the Hami melon, in the squatting seedling root extension period of the Hami melon in 5 days in 6 months and 5 days, the organic trace element nutrient is diluted by adding water by 300 times at 0.1 kg/mu for the first time and is sprayed to the leaf surface of the Hami melon in 7 months and 7 days, the bud development and flowering period of the Hami melon is realized, the organic trace element nutrient is diluted by adding water by 300 times at 0.1 kg/mu for the second time and is sprayed to the leaf surface of the Hami melon in 25 days in 7 months, and the organic trace element nutrient is diluted by adding water by 300 times at 0.1 kg/mu for the third time and is sprayed to the leaf surface of the Hami melon in the expansion period.

When the Liaodang is biologically prevented and treated, the Hami melon 86-1 is planted to prevent diseases and insects.

Comparative example 4

The hami melon 86-1 is planted by conventional fertilization operation, the nitrogen, phosphorus and potassium which are purchased in the market at a ratio of 20:20:20 are applied to the hami melon at a ratio of 85 kg/mu, and seedlings emerge through dripping water.

The growth of field Hami melon brook and Hami melon was examined at each drip irrigation as shown in tables 8 and 9.

Table 8 questionnaire of field growth conditions for example 2 and comparative example 4 cantaloupe

Table 9 comparison of cantaloupe yields in example 2 and comparative example 4

As can be seen from tables 8 and 9, the soil conditioner, the microbial agent, the soil fertility balancing agent and the organic trace element nutrient are used in combination, so that the salt and alkali can be effectively reduced, the soil hardening state can be improved, the soil activity can be stimulated, the soil fertility can be improved, and the soil organic matter can be improved; and the soil conditioner and the microbial agent are used in a matched manner, salt and alkali are discharged deeply, the effect of biological enzyme generated by microorganisms on inhibiting the Hami melon broomrape reaches 94%, the test field seedlings are full and uniform, the emergence rate reaches 97%, the flowering is early, the Hami melon grows rapidly, stems are thick and strong, leaves are dark green, the flowering is early, the Hami melon ripens in advance, and no diseases or insect pests exist, so that the soil conditioner and the microbial agent play a role, the emergence rate of the comparative example 4 is only 85%, the emergence is not neat, the flowering is late, the maturity is late, the occurrence of sclerotinia is particularly serious, the broomrape death rate is 0, each broomrape seed is full, the broomrape has fleshy roots and is plump, and the broomrape seeds fall on the ground to wait for hosts. Compared with the comparative example 4, the soil of the embodiment of the invention has the advantages of good condition, looseness, easy ploughing, low rate of vacant shell, high maturing rate, high yield and 21.2% of yield increase.

In order to verify the effect of continuous planting on the land where the orobanche occurs in great occurrence in the embodiments of the present invention, the same-field planting of hami melons 86-1 in the embodiments of the present invention is continued for 7 years of continuous cropping fixed-point demonstration from 4 months to 9 months in 2019 in 2014, and soil parameters and yield changes are evaluated, as shown in table 10.

Table 10 table of soil parameters, broomrape growth and variation in cantaloupe yield before and after 7 years of continuous cropping of cantaloupe in example 2

As can be seen from table 10, after continuous cropping, the pH value of the soil decreases year by year, and after 2019, the pH value of the soil is reduced to 7.3, which is close to neutral, and reaches the range of optimal crop growth; the salt content of the soil also decreases year by year, and is as low as 0.51 percent after the soil is applied in 2019, which indicates that the deposited salt of the soil can be utilized and absorbed by crops in a continuous cropping state, and is converted into nutrient substances, so that the nutrient is provided for the yield increase of the Hami melons, and the using amount of chemical fertilizers is reduced; the soil organic matter rises year by year, reaches the maximum in 2019, and greatly rises from the 7 th year to reach 1.09%, so that the soil is fundamentally improved after being planted by the method for a long time, and the sustainable utilization of the soil is realized; beneficial floras such as applied microbial agents and the like are continuously propagated at the roots of the Hami melons, so that the Roche is died in advance, the incidence rate of the Roche in 2019 is 0.7% in the embodiment of the invention, the incidence rate of the Roche in the plot of comparative example 4 is 100%, almost every Hami melon is parasitized, so that the plants are short, small and weak, the infection rate is high, and the Hami melons can not be continuously planted in the plot of comparative example 4; according to the embodiment of the invention, the yield of the Hami melons planted in the plots is stable, the yield is increased by more than 20.6% compared with that of a comparative example 4, the highest yield reaches 33.8% in 2019, and the Hami melons planted in the plots of the comparative example 4 have no commodity and are even out of the limit.

Examples 3-1 to 3-4

Basic conditions of the test foundation: test sites were set in 6 groups of Barun Harr Modun Tourman village (example 3-1, comparative example 5-1) in Hexi county, 1 group of Baerhai Daisei village (example 3-2, comparative example 5-2) in Yan county, 5 groups of Beijing village (example 3-3, comparative example 5-3) in Yan county, and 2 groups of Queen village in Queen county in Bohu county (example 3-4, comparative example 5-4) in Pepper lands, each of 10 acres; the soil is moisture soil and has medium fertility; the test time is 4-9 months in 2014.

And (3) trial article species: no. 7, a local agricultural resource, provided by a Pin part.

(1) Improving saline and alkaline soil: according to the raw material components and the weight parts and contents of the raw material components in the examples 3 of tables 1 and 2, when drip irrigation is carried out for the first time in the seedling returning period of 4 plots after transplanting linear peppers respectively in the 28 th, 3 rd, 25 th and 11 th days of 4 months, 5 months and 5 months, the soil conditioner is drip-irrigated into soil by 3 kg/mu for the first time, the linear peppers are extended into roots by 16 th months, the soil conditioner is drip-irrigated into soil by 3 kg/mu for the second time, and the linear peppers are flowering by 6 th months, the soil conditioner is drip-irrigated into soil by 3 kg/mu for the third time, so that the saline-alkali improvement of the soil is carried out;

(2) the microbial bacteria control broomrape: according to the components, the weight parts and the contents of the raw materials in the examples 3 shown in the tables 1 and 3, in 4 plots respectively, when the linear peppers are subjected to secondary drip irrigation in the root extension period of the linear peppers at 16 days in 6 months after the seedling return period is transplanted, the microbial inoculum is firstly dripped into the soil at 3 kg/mu, in the flowering period of the linear peppers at 6 days in 7 months, the microbial inoculum is secondly dripped into the soil at 4 kg/mu, in the fruit expansion period of the linear peppers at 20 days in 7 months, the microbial inoculum is thirdly dripped into the soil at 4 kg/mu, and the microbial inoculum production bio-enzyme control is listed;

(3) and (3) soil fertility balance adjustment: according to the raw material components and the weight parts of the raw material components in the examples 3 in the tables 1 and 4, when the second drip irrigation is carried out on 4 plots and the seedling returning period of the transplanted linear peppers is in the root extending period of the linear peppers at 16 days in 6 months, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 15 kg/mu, 2 kg/mu and 1 kg/mu for the first time, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 30 kg/mu, 2 kg/mu and 0.5 kg/mu for the second time in the flowering period of the linear peppers at 6 days in 7 months, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively dripped into the soil at 35 kg/mu, 2 kg/mu and 0.5 kg/mu for the third time in the fruit expanding period of the linear peppers at 20 days in 7 months, and the soil fertility balance adjustment is carried out;

in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 45 min; after drip irrigation with the soil fertility balancing agent, drip irrigation for 1.5h with clear water;

(4) leaf surface nutrition supplement: according to the raw material components, the weight parts and the contents of the raw material components in example 3 of tables 1 and 5, in 4 plots respectively, during the growth period of linear peppers, after the linear pepper extends to the root at 15 days in 6 months, the organic trace element nutrient is diluted by 0.15 kg/mu with water for 300 times and sprayed to the leaf surfaces of crops for the first time, during the flowering period of linear peppers at 6 days in 7 months, the organic trace element nutrient is diluted by 0.15 kg/mu with water for 300 times and sprayed to the leaf surfaces of crops for the second time, and during the fruit expanding period of linear peppers at 20 days in 7 months, the organic trace element nutrient is diluted by 0.1 kg/mu with water for 300 times and sprayed to the leaf surfaces of crops for the third time, so that the nutrition supplement is carried out.

When the Liaodong Liang is biologically prevented and treated, the No. 7 linear pepper is planted to prevent diseases and insects.

Comparative examples 5-1 to 5-4

No. 7 linear peppers are planted in the 4 plots respectively, and conventional fertilization operation is carried out.

And (3) inspecting the change of soil parameters before and after the linear peppers are planted, the field occurrence condition of the linear peppers and the quality and yield of the linear peppers, as shown in tables 11-13.

TABLE 11 Table of changes in soil parameters before and after planting of Zanthoxylum piperitum of examples 3-1 to 3-4 and comparative examples 5-1 to 5-4

TABLE 12 questionnaire of the field occurrence of the litsea sericea in examples 3-1 to 3-4 and comparative examples 5-1 to 5-4

TABLE 13 quality and output scales for cayenne peppers of examples 3-1 to 3-4 and comparative examples 5-1 to 5-4

From tables 11 to 13, it can be seen that the soil conditioner, the microbial agent, the soil fertility balancing agent and the organic trace element nutrient are used in combination in the embodiment of the invention, so that the salt and alkali can be effectively reduced, the soil hardening state can be improved, the soil activity can be excited, the soil fertility can be improved, and the soil organic matter can be improved; the soil conditioner and the microbial agent are used in a matched manner, the organic acid complexes soil cations, and the microorganisms decompose salt ions, so that the harm of salt and alkali to crops is reduced, the environment of soil near the root system of the plants is improved, the root system of the linear peppers is in a neutral and acidic environment, the permeability of nutrient substances is improved, the speed of supplementing nutrients to soil microorganisms is increased, the metabolic activity of the soil microorganisms is enhanced, the growth of the crops is promoted, the broomrape growth is inhibited, and the effect of inhibiting the linear peppers by biological enzymes generated by the microorganisms is as high as 95.3% on average; meanwhile, the fruit number and the weight average of single fruit of each plant of the linear pepper in the embodiment of the invention are higher than those of the linear pepper in the comparative example 5, the yield is averagely up to 74.4 percent, the linear pepper is respectively increased by 1480.29 yuan/mu, 1235.19 yuan/mu, 1232.53 yuan/mu and 1142.66 yuan/mu according to the calculation of 1.9 yuan/kg, the cost of removing the application is 569.5 yuan/mu, the gains of 4 test demonstration points in the embodiment of the invention are 910.79 yuan/mu, 665.69 yuan/mu, 663.03 yuan/mu and 573.16 yuan/mu, and the average gain is 703.16 yuan/mu. Therefore, the embodiment of the invention improves the soil salinity and alkalinity, improves the yield, reduces the fertilizer usage amount and exerts the maximum benefits of the fertilizer and water resources while preventing and controlling the Roche.

Example 4

Basic conditions of the test foundation: the test land is arranged in a 5-bucket No. 5 strip field of Yicar village arrangement area of four counties of Homozuke, Ili, Xinjiang, and has a control area of 5 mu, the test land is a flat land, the soil type is brown soil, and the soil texture is loam soil; the previous crop is corn, the yield of the previous crop is 980 kg/mu, and the fertilizing amount of the previous crop is as follows: n: 21.05 kg/mu, P₂O₅: 10.25 kg/mu, K₂O: 5.25 kg/mu; and (3) soil analysis results: 17.63g/kg of organic matter, 50mg/kg of quick-acting nitrogen, 12mg/kg of available phosphorus, 170mg/kg of quick-acting potassium, 7.87 of pH value and 0.48% of salt content of soil; the test time is 2015 years for 5-10 months.

And (3) trial article species: melon 86-1, available from local agricultural companies.

(1) Improving saline and alkaline soil: according to the components, the weight parts and the contents of the raw materials in the examples 4 in the tables 1 and 2, when seedling emergence water is subjected to drip irrigation for the first time in 5-month and 10-day days after melon sowing, the soil conditioner is dripped into soil at 6 kg/mu for the first time, the melon extends to the root in 5-month and 25-day days, the soil conditioner is dripped into the soil at 6 kg/mu for the second time, the melon blooms in 6-month and 10-day for a long time, and the soil conditioner is dripped into the soil at 8 kg/mu for the third time, so that the saline-alkali soil is improved;

(2) the microbial bacteria control broomrape: according to the raw material components and the weight parts and contents of the components and the contents of the components in the examples 4 in the tables 1 and 3, when drip irrigation is carried out for the second time in the root extending period of the melon in 25 days and 5 months after sowing the melon, the microbial inoculum is drip-irrigated into the soil for the first time at 2 kg/mu, the melon blooms for the long time in 10 days and 6 months, the microbial inoculum is drip-irrigated into the soil for the second time at 4 kg/mu, the melon is drip-irrigated into the soil for the second time in the fruit setting period of the melon in 25 days and 6 months, the microbial inoculum is drip-irrigated into the soil at 4 kg/mu for the third time, the melon is swell period in 10 days and 7 months, the microbial inoculum is drip-irrigated into the soil at 3 kg/mu for the fourth time in the mature period of the melon in 25 days and 7 months, and the microbial;

(3) and (3) soil fertility balance adjustment: according to the raw material components and the weight parts of the components in the examples 4 in the tables 1 and 4, when drip irrigation is carried out for the second time in the root extending period of the melon in 25 days in 5 months after sowing the melon, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into soil in 10 kg/mu, 1 kg/mu and 1 kg/mu for the first time, the melon flowers are flourishing for a long time in 10 days in 6 months, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into soil in 15 kg/mu, 1 kg/mu and 0.2 kg/mu for the second time, the nitrogen-phosphorus-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into soil in 22 kg/mu, 2 kg/mu and 0.3 kg/mu in 25 days in 6 months in the fruit setting period of the melon in 25 days in 6 months, the nitrogen-potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into soil in 20 kg/mu, 2 kg/mu and 0, 0.3 kg/mu is dripped into soil, in the mature period of the melon in 25 days in 7 months, the nitrogen, phosphorus and potassium fertilizers, the secondary element fertilizers and the trace element fertilizers are respectively dripped into the soil in 23 kg/mu, 1 kg/mu and 0.2 kg/mu in the fifth time, and the soil fertility balance is adjusted;

in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 45 min; after drip irrigation with the soil fertility balancing agent, drip irrigation for 1.5h with clear water;

(4) leaf surface nutrition supplement: according to the components, the weight parts and the contents of the raw materials in the examples 4 of tables 1 and 5, in the root extending period of the melons in 25 days in 5 months, the organic trace element nutrient is diluted by adding water by 300 times at 0.15 kg/mu for the first time and is sprayed to the leaf surfaces of crops in the fruit setting period of the melons in 25 days in 6 months, the organic trace element nutrient is diluted by adding water by 300 times at 0.15 kg/mu for the second time and is sprayed to the leaf surfaces of the crops in the fruit setting period of the melons in 25 days in 7 months, and in the expanding period of the melons in 10 days in 7 months, the organic trace element nutrient is diluted by adding water by 300 times at 0.2 kg/mu for the third time and is sprayed to the leaf surfaces of.

When the Liaodong is biologically controlled, the muskmelon 86-1 is planted to prevent diseases and insects.

Comparative example 6

The melon is planted by the conventional fertilization operation and the same amount of clear water is applied.

The field melon and melon broomrape (8 months and 3 days 2015) were examined for growth as shown in table 14.

TABLE 14 production of melons and field growth of melon broomrape questionnaires for example 4 and comparative example 6

As can be seen from table 14, in the embodiment of the present invention, the soil conditioner, the microbial agent, the soil fertility balancing agent, and the organic trace element nutrient are used in combination, so that the salt can be effectively reduced, the alkali can be removed, the hardened state of the soil can be improved, the soil activity can be activated, the soil fertility can be improved, and the soil organic matter can be improved; the soil conditioner and the microbial agent are matched for use, salt and alkali are discharged to the deep, and the effect of biological enzyme generated by the microorganisms on inhibiting the muskmelon broomrape reaches 99%; meanwhile, the yield of the melons reaches 30%, the pure yield per mu of the muskmelon is 2845.2 yuan, the pure income of the comparative example 6 is 2190 yuan/mu, and the yield of the muskmelon is 655.2 yuan/mu compared with the comparative example 6; and the maturation period of the inventive example was advanced by 7 days compared to comparative example 6. Therefore, the embodiment of the invention improves the saline-alkali degree of the soil, prevents and treats the broomrape, improves the yield, reduces the using amount of the chemical fertilizer and exerts the maximum benefit of water and fertilizer resources.

Examples 5-1 to 5-3

Basic conditions of the test foundation: the test site is set in 1 group of Yiwu county paste in Mao lake town Yinmeiluramura 1 in Hami area of Xinjiang, farmers who cook hot wood and the area is 5 mu (embodiment 5-1 and comparative example 7-1); wu county paste the Rou lake town xi Kai Er village 4 groups, peasant households with AbuDou tone, area 10 mu (embodiment 5-2, comparative example 7-2); wu county paste 5 groups of Mao lake town development area village, farmer Mao Yong, 185 mu area (example 5-3, comparative example 7-3); the soil is moisture soil, the soil fertility is moderate, the first-class land is smooth, the management measures are proper, and the film is mechanically paved; the soil texture is light soil, the soil fertility organic matter before planting is 20.3mg/kg, the alkaline hydrolysis nitrogen is 62mg/kg, the quick-acting phosphorus is 9mg/kg, and the quick-acting potassium is 110 mg/kg; after planting, the soil fertility organic matter is 19.8mg/kg, the alkaline hydrolysis nitrogen is 66mg/kg, the quick-acting phosphorus is 11mg/kg, and the quick-acting potassium is 102 mg/kg; the first crop is wheat, and the yield is 450 kg/mu; the test time is 5-8 months in 2013.

And (3) trial article species: hami melon (New Mi 15), available from local agricultural company.

(1) Improving saline and alkaline soil: according to the components, the weight parts and the contents of the raw materials in the examples 5 of tables 1 and 2, when seedling emergence water is subjected to drip irrigation for the first time in 17 days after 5 months and 5 months after seeding of Hami melons in 3 plots, the soil conditioner is dripped into soil by 5 kg/mu for the first time in the squatting stage of Hami melons in 10 days in 6 months, 15 days in 6 months and 16 days in 6 months, the root extension stage of Hami melons in 6 months, the soil conditioner is dripped into soil by 5 kg/mu for the second time in the flowering stage of Hami melons in 20 days in 6 months, 25 days in 6 months and 26 days in 6 months, the soil conditioner is dripped into soil by 7 kg/mu for the third time in the setting stage of Hami melons in 1 day in 7 months, 6 days in 7 months and 7 days in 7 months, and the soil conditioner is dripped into soil by 8 kg/mu for the fourth time to improve the saline and alkaline of the soil;

(2) the microbial bacteria control broomrape: according to the raw material components and the weight parts and contents of the raw material components in the examples 5 of tables 1 and 3, when the hami melon is subjected to secondary drip irrigation in the 6-month 10-day, 6-month 15-day and 6-month 16-day crouching and rooting periods after the hami melon is sown in 3 land blocks respectively, 3 kg/mu of microbial inoculum is dripped into soil for the first time in the 6-month 10-day, 6-month 15-day and 6-month 16-day crohning periods respectively, 20-month 6-day, 6-month 25-day and 6-month 26-day hami melon flowering periods respectively, 5 kg/mu of microbial inoculum is dripped into soil for the second time in the 7-month 1-day, 7-month 6-day and 7-month 7-day hami melon fruit setting periods respectively, 6 kg/mu of microbial inoculum is dripped into soil for the third time, 7-month 15-day and 7-month 16-day turfs of hami melons are respectively, 6 kg/mu of microbial inoculum is dripped into;

(3) and (3) soil fertility balance adjustment: according to the raw material components and the weight parts of the components in the examples 5 of tables 1 and 4, when the hami melons are sown in 3 land blocks and are respectively drip-irrigated for the second time in the 6-month 10-day, 6-month 15-day and 6-month 16-day croaker squat root extension periods, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into the soil for the first time at 15 kg/mu, 2 kg/mu and 2 kg/mu at the 20-day, 6-month 25-day and 6-month 26-day of the hami melons respectively, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-irrigated into the soil for the second time at 20 kg/mu, 3 kg/mu and 1 kg/mu at the 20-day, 6-month and 7-day of the hami melons at the 6-month and 6-month, 7-day of the hami melons at the second time, the nitrogen phosphorus potassium fertilizer, the medium element fertilizer and the trace element fertilizer are respectively drip-mu at 25 kg/mu, 2kg/, in the Hami melon expansion periods of 10 days in 7 months, 15 days in 7 months and 16 days in 7 months respectively, the nitrogen, phosphorus and potassium fertilizers, the medium element fertilizers and the trace element fertilizers are respectively dripped into the soil by 25 kg/mu, 3 kg/mu and 1.4 kg/mu for the fourth time, and the soil fertility balance adjustment is carried out;

in the same drip irrigation, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 45 min; after drip irrigation with the soil fertility balancing agent, drip irrigation for 1.5h with clear water;

(4) leaf surface nutrition supplement: according to the components, the weight parts and the contents of the raw materials in the examples 5 of tables 1 and 5, in 3 land blocks and in the growth period of Hami melons, the squatting seedlings of Hami melons are respectively sprayed to the leaf surfaces of crops in the 10 th 6 month, 15 th 6 month and 16 th 6 month and the root stretching period of Hami melons, the organic trace element nutrient is diluted by 0.2 kg/mu of water for 300 times for the first time, the hami melons are respectively sprayed to the leaf surfaces of the crops in the 20 th 6 month, 25 th 6 month and 26 th 6 month, the organic trace element nutrient is diluted by 0.2 kg/mu of water for 300 times for the second time, the hami melons are respectively sprayed to the leaf surfaces of the crops in the 1 th 7 month, 6 th 7 month and 7 th month 7 day, and the organic trace element nutrient is sprayed to the leaf surfaces of the crops in the setting period of Hami melons in the third time, and the leaf surface nutrition supplement is carried.

When the orobanche is biologically prevented and controlled, the cantaloupe (New Mi No. 15) is planted to prevent diseases and insects.

Comparative examples 7-1 to 7-3

Hami melons are planted in the 3 land parcels respectively, conventional fertilization operation is carried out, nitrogen, phosphorus and potassium are 150 kg/mu, trace elements are 10 kg/mu, and organic fertilizers are 50 kg/mu.

Field management: covering the film 5 months and 5 days, and simultaneously using diammonium phosphate and potassium sulfate as base fertilizers; sowing the seeds for 20 days in 5 months, wherein the row spacing is 0.4 m, the row spacing is 3m, 2-3 seeds are sowed in each hole, and the number of basic seedlings is 1000 seedlings per mu; watering 5 times and intertillage 3 times in the whole growth period; spraying 200 g/mu of monopotassium phosphate and 50 mL/mu of amino acid foliar fertilizer on the leaf surface of the leaf surface.

The field occurrence of the hami melons and broomrape, and the quality and yield of the hami melons are examined as shown in tables 15 and 16.

TABLE 15 questionnaire of field occurrence of examples 5-1 to 5-3 and comparative examples 7-1 to 7-3 Hami melon orobanche

As is clear from Table 15, the fertilizer application time of examples 5-1 to 5-3 was early, the incidence of Hami melon orobanche was late, and the number of incidence of the fertilizer was only 2 to 3 plants/m in the 7 th, 18 th day of investigation²The prevention rate is about 90%, which shows that the soil conditioner, the microbial agent, the soil fertility balancing agent and the organic trace element nutrient are matched to inhibit the growth of the broomrape and play a role in preventing and treating the broomrape in the embodiment of the invention.

TABLE 16 Hami melon quality and yield scales for examples 5-1 to 5-3 and comparative examples 7-1 to 7-3

As can be seen from Table 16, the refractive sugar contents of Hami melons in examples 5-1 to 5-3 of the present invention are 15.4%, 15.3%, and 15.6%, respectively, which are higher than those in comparative examples 7-1 to 7-3 by 0.5%, 0.3%, and 0.4%, respectively, and the higher the refractive sugar content, the more the Hami melons have the same weight; the average single melon weight of Hami melons in examples 5-1 to 5-3 of the invention is respectively 0.5kg, 0.76kg and 0.95kg higher than that of comparative examples 7-1 to 7-3; the color difference of the Hami melons in the embodiments 5-1 to 5-3 is respectively higher than that in the comparative examples 7-1 to 7-3 by 0.45a/b, 0.61a/b and 0.47a/b, and the higher the color difference of the Hami melons is, the brighter the Hami melons are; compared with the yield of the comparative examples 7-1 to 7-3, the yield of the invention in the examples 5-1 to 5-3 is increased by 417 kg/mu, 421 kg/mu and 672 kg/mu respectively, the yield is increased by 30.0 percent, and the obvious yield increasing effect is achieved; while the using amount of the fertilizer which is conventionally operated is 150 kg/mu, the applying amount of the fertilizer of the embodiment of the invention is only 100.4 kg/mu, and the using amount of the fertilizer is reduced by 49.6 kg/mu. The embodiment of the invention combines the soil conditioner, the microbial agent, the soil fertility balancing agent and the organic trace element nutrient for use, improves the yield, improves the quality of the Hami melon, reduces the using amount of the chemical fertilizer and exerts the maximum benefit of water and fertilizer resources while preventing and treating the Roche.

Claims (9)

1. The composition for biologically controlling orobanche coerulescens is characterized by mainly comprising the following components: soil conditioner, microbial agent, soil fertility balancing agent and organic trace element nutrient.

2. The composition for biologically controlling orobanche coerulescens according to claim 1, wherein the weight parts of the components are as follows: 1-30 parts of soil conditioner, 2-30 parts of microbial agent, 80-150 parts of soil fertility balancing agent and 0.15-0.60 part of organic trace element nutrient.

3. The biological broomrape-controlling composition according to claim 1 or 2, characterized in that: the soil conditioner comprises the following main components in percentage by weight: 110-180 g/L of amino acid, 210-280 g/L of fulvic acid and the pH value of 2.5-4.5;

the microbial agent is mainly prepared by mixing a microbial agent and a carrier in a mass ratio of 1: 1-70; the viable count of the microbial inoculum is 1.0 multiplied by 10⁸～1.0×10¹⁰Per gram; the microbial preparation is one or more of a trichoderma viride preparation, a bacillus licheniformis preparation, a bacillus subtilis preparation, a saccharomyces cerevisiae preparation, a bacillus amyloliquefaciens preparation, a bacillus laterosporus preparation, a bacillus mucilaginosus preparation or a pseudomonas palustris preparation; the carrier is one or more of attapulgite, bentonite, potassium feldspar, amino acid, fulvic acid, humic acid or alginic acid;

the soil fertility balancing agent mainly comprises a nitrogen-phosphorus-potassium fertilizer, a secondary element fertilizer and a trace element fertilizer; the fertilizer comprises the following components in parts by weight: 75-120 parts of nitrogen-phosphorus-potassium fertilizer, 4-20 parts of medium element fertilizer and 1-10 parts of trace element fertilizer; in the nitrogen-phosphorus-potassium fertilizer, the nitrogen fertilizer, the phosphorus fertilizer and the potassium fertilizer are as follows in parts by weight: 1-30 parts of nitrogenous fertilizer, 1-40 parts of phosphate fertilizer and 1-40 parts of potash fertilizer; the nitrogen fertilizer is one or more of urea, ammonium sulfate, ammonium nitrate or ammonium chloride; the phosphate fertilizer is one or more of ammonium dihydrogen phosphate, ammonium phosphate nitrate or urea phosphate; the potash fertilizer is one or more of potassium sulfate, potassium chloride, potassium nitrate or potassium dihydrogen phosphate; in the secondary element fertilizer, the secondary element fertilizer and the weight parts thereof are respectively as follows: 1-20 parts of calcium fertilizer, 1-30 parts of magnesium fertilizer and 1-10 parts of silicon fertilizer; the calcium fertilizer is one or more of calcium nitrate, calcium chloride, calcium sulfate or organic acid chelated calcium; the magnesium fertilizer is one or more of magnesium nitrate, magnesium chloride, magnesium sulfate or organic acid chelated magnesium; the silicon fertilizer is one or more of sodium silicate, potassium silicate or organic silicon; in the trace element fertilizer, the trace element fertilizer comprises the following components in parts by weight: 0.5-4.0 parts of zinc fertilizer, 0.1-1.0 part of manganese fertilizer, 0.1-0.4 part of boron fertilizer, 0.1-0.4 part of copper fertilizer, 0.5-8.0 parts of iron fertilizer, 0.01-0.30 part of molybdenum fertilizer, 0.04-0.40 part of rare earth fertilizer, 0.01-0.05 part of cobalt fertilizer and 0.01-0.20 part of selenium fertilizer; the zinc fertilizer is one or more of zinc sulfate, zinc nitrate, zinc chloride or organic acid chelated zinc; the manganese fertilizer is one or more of manganese sulfate, manganese nitrate, manganese chloride or organic acid chelated manganese; the boric fertilizer is borax and/or boric acid; the copper fertilizer is one or more of copper sulfate, copper nitrate, copper chloride or organic acid chelated copper; the iron fertilizer is one or more of ferrous sulfate, ferric nitrate, ferric chloride or organic acid chelated iron; the molybdenum fertilizer is ammonium molybdate and/or sodium molybdate; the cobalt fertilizer is cobalt sulfate; the selenium fertilizer is sodium selenite; the organic acid is one or more of ethylenediamine tetraacetic acid, amino acid or citric acid;

the organic trace element nutrient comprises the following main components in percentage by weight: 130-300 g/L amino acid, 1-9 g/L calcium, 1-9 g/L magnesium, 0.02-9 g/L zinc, 0.02-9 g/L manganese, 0.005-5 g/L boron, 0.005-9 g/L copper, 0.02-9 g/L iron, 0.001-1 g/L molybdenum and 0.001-0.1 g/L selenium.

4. A method for the biological control of orobanche coerulescens using a composition according to any one of claims 1 to 3, comprising the steps of:

(1) improving saline and alkaline soil: dripping a soil conditioner into soil from the first drip irrigation after sowing or transplanting to improve the saline and alkaline of the soil;

(2) the microbial bacteria control broomrape: dripping the microbial agent into soil from the second drip irrigation after sowing or transplanting, and controlling broomrape by the biological enzyme generated by the microbial agent;

(3) and (3) soil fertility balance adjustment: from the second drip irrigation after sowing or transplanting, drip irrigation the soil fertility balancing agent into the soil for soil fertility balance adjustment;

(4) leaf surface nutrition supplement: and during the growth period of the crops, the organic trace element nutrient is sprayed to the leaf surfaces of the crops to supplement the nutrition of the leaf surfaces.

5. The method for biologically controlling orobanche according to claim 4, wherein: in the step (1), the crops are one or more of watermelon, Hami melon, hot pepper, melon, sunflower, watermelon, pea, broad bean, carrot, celery, tobacco, flax or tomato; the total dosage of the soil conditioner is 1-30 kg/mu; 1-7 kg/mu of soil conditioner is subjected to drip irrigation for the first time, the interval of each drip irrigation is 10-50 days, and the drip irrigation is divided into more than or equal to 2 times.

6. The method for biologically controlling orobanche according to claim 4 or 5, wherein: in the step (2), the total dosage of the microbial inoculum is 2-30 kg/mu; 1-3 kg/mu of microbial agent is subjected to primary drip irrigation, the interval of each drip irrigation is 6-21 days, and the total drip irrigation is more than or equal to 3 times.

7. A method for biologically controlling Oroban according to any one of claims 4 to 6, wherein: in the step (3), the total dosage of the soil fertility balancing agent is 80-150 kg/mu, wherein 75-120 kg/mu of nitrogen-phosphorus-potassium fertilizer, 4-20 kg/mu of medium element fertilizer and 1-10 kg/mu of trace element fertilizer; 2-15 kg/mu of nitrogen-phosphorus-potassium fertilizer, 1-3 kg/mu of medium element fertilizer and 0.5-2.0 kg/mu of trace element fertilizer are subjected to drip irrigation for the first time, the drip irrigation interval is 6-21 days, and the drip irrigation is divided into more than or equal to 3 times.

8. A method for biologically controlling Oroban according to any one of claims 4 to 7, wherein: in the step (4), the total dosage of the organic trace element nutrient is 0.15-0.60 kg/mu; the organic trace element nutrient is sprayed on the leaf surfaces for 1 to 3 times, and the spraying interval is10 to 35 days every time.

9. A method for biologically controlling Oroban according to any one of claims 4 to 8, wherein: in the steps (1) - (4), when the soil conditioner, the microbial agent and the soil fertility balancing agent are subjected to drip irrigation at the same time, the soil conditioner, the microbial agent and the soil fertility balancing agent are sequentially applied in sequence, and the application interval time is 30-60 min.