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Ecometabolomics Studies of Bryophytes

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Bioactive Compounds in Bryophytes and Pteridophytes

Part of the book series: Reference Series in Phytochemistry ((RSP))

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Abstract

Bryophytes are the largest group of non-vascular plants that occur in almost any land ecosystem and have remarkable impact on ecosystem functioning at a global level. Despite that they have evolved an extraordinary chemical diversity, only a few bryophytic species have been studied using metabolomic techniques. Ecometabolomics systematically investigates the composition of metabolic compounds in bryophytes and relates these to organismal and environmental interactions. The application of ecometabolomics to bryophytic organisms can lead to new insights into their molecular biology, can identify novel bioactive natural products, can shed light on the phylogenetic and evolutionary mechanisms bryophytes realize in order to sustain ecological change, or can greatly improve the mechanistic understanding of ecological processes that are mediated by metabolic compounds at various levels. In this chapter, we first describe ecometabolomics and provide an introduction to how it can be performed. We then focus on case studies covering the various research fields of natural product chemistry, chemodiversity, chemotaxonomy/chemophenetics, functional ecology and plant traits, bioindication and biomonitoring, bioactivities, and the molecular biology of bryophytes. Finally, we present the latest advancements in analytic and computational methods to show the tremendous potential of the emerging technology of ecometabolomics for research with bryophytic organisms.

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Abbreviations

(C)CA:

(Canonical) Correspondence Analysis

(N)MDS:

(Non-metric) Multi-Dimensional Scaling

4CL:

4-coumarate CoA ligase

ACP:

Acyl carrier protein

ANOVA:

Analysis of Variances

C:

Net plant carbon

C4H:

Cinnamate 4-hydrolase

CAWG:

Chemical Analysis Working Group

CBGA:

Cannabigerolic acid

CHS:

Chalcone synthase

CoA:

Coenzyme A

DBR:

Double bond reductase

DDA:

Data-dependent acquisition

DIA:

Data-independent acquisition

FAIR:

Findable, Accessible, Interoperable, Reusable

FT-ICR-MS:

Fourier Transform Ion Cyclotron Resonance Mass-Spectrometry

GC:

Gas chromatography

GC/MS:

Gas chromatography coupled to mass-spectrometry

H′:

Shannon diversity index

IPP:

Isopentyl diphosphate delta isomerase

J:

Pielou’s evenness

LC:

Liquid chromatography

LC/MS-MS:

liquid chromatography coupled with tandem mass-spectrometry

m/z:

Mass-to-charge ratio

MEP:

Non-mevalonate

MS:

Mass-spectrometry

MSI:

Metabolomics Standards Initiative

MTPSL:

Microbial terpene synthase-like

MVA:

Mevalonate

N:

Nutrients

NMR:

Nuclear magnetic resonance

PAL:

Phenylalanine ammonia-lyase

PCA:

Principal Component analyses

PDH:

Pyruvate dehydrogenase

PLS:

Partial Least Squares regression

PLSDA:

PLS coupled with Discriminant Analysis

QC:

Quality control

RDA:

ReDundancy Analysis

S:

Compound richness

SOM:

Soil organic matter

STCS:

Stilbene carboxylate synthase

THC:

Tetrahydrocannabinol

TIMS:

Ion-Mobility Mass-Spectrometry

ToF:

Time of flight

U:

Number of unique compounds

VOCs:

Volatile organic compounds

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Acknowledgments

KP, YP, and HU acknowledge the support of iDiv (funded by the German Research Foundation, DFG-FZT 118, 202548816). KBJ was funded by NSERC via the CGS-MSFSS (Application No. 566822-2021). Further, we like to thank the Leibniz Foundation for supporting this study. Lastly, we like to thank Harald Zechmeister for providing valuable feedback and for improving the manuscript.

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Authors and Affiliations

  1. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany

    Kristian Peters, Yvonne Poeschl & Henriette Uthe

  2. Institute of Biology/Geobotany and Botanical Garden, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany

    Kristian Peters

  3. Bioinformatics and Scientific Data, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany

    Kristian Peters & Kaitlyn L. Blatt-Janmaat

  4. Institute of Biodiversity, Friedrich Schiller University, Jena, Germany

    Yvonne Poeschl & Henriette Uthe

  5. Department of Chemistry, University of New Brunswick, Fredericton, NB, Canada

    Kaitlyn L. Blatt-Janmaat

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  1. Kristian Peters
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  4. Henriette Uthe
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Correspondence to Kristian Peters .

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Editors and Affiliations

  1. Department of Botany, Karnatak University, Dharwad, Karnataka, India

    Hosakatte Niranjana Murthy

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Peters, K., Poeschl, Y., Blatt-Janmaat, K.L., Uthe, H. (2023). Ecometabolomics Studies of Bryophytes. In: Murthy, H.N. (eds) Bioactive Compounds in Bryophytes and Pteridophytes. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-031-23243-5_30

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