Abstract
In vitro propagation protocol was developed for Saussurea lappa (Clarke.) species threatened by over exploitation due to medicinal importance and habitat destruction in Ladakh region of India. The aim of the present study was to examine the main aspects of in vitro callus induction (CI) and plantlet regeneration of S. lappa. Explants were cultured on Murashige and Skoog (MS) basal medium supplemented with various concentrations and combinations of auxins and cytokinins for in vitro CI and plantlets regeneration. Callus initiation and induction was observed within eight and fifteen days after inoculation. 3 mg/L 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and 5 mg/L Kinetin (Kin) was found to be more effective for CI frequencies in all the explants as compared to other combinations. Root (79.6 ± 0.8) and stem (76.4 ± 0.6) explants were found with better callus frequencies and early response as compared to other explants. Maximum number of shoots (11.8 ± 0.7), highest shoot length (9.2 ± 0.6 cm), maximum root number (6.2 ± 0.7) and highest root length (6.6 ± 0.8 cm) were found on MS medium with 2 mg/L Kinetin (Kin) and 2 mg/L Indole-3-butyric-acid in root derived callus explant. Plantlets with 3–5 shoots were transferred to potting mixture containing sand: soil: perlite (1:1:1) for acclimatization to field conditions and further multiplication. For the first time, protocol has been developed in S. lappa for in vitro CI and plantlet regeneration that holds robust potential for metabolite production and large-scale propagation.
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Authors are thankful to Defence Research and Development Organization (DRDO), Ministry of Defence, Government of India for providing financial assistance for the research.
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Warghat, A.R., Bajpai, P.K., Rewang, S. et al. In Vitro Callus Induction and Plantlet Regeneration of Saussurea lappa (Clarke.) from Ladakh Region of India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 651–660 (2016). https://doi.org/10.1007/s40011-015-0496-y
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DOI: https://doi.org/10.1007/s40011-015-0496-y