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Fermenting and Lignin Degradability of a White-Rot Fungus Coriolopsis trogii Using Industrial Lignin as Substrate

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Abstract

Bio-depolymerized the lignin macromolecules into low molecular lignin-derived aromatic compounds satisfies the requirement for carbon dioxide peaking and is also one of the important ways to realize lignin valorization. Coriolopsis trogii is a kind of less reported lignin-degrading white-rot fungus. The degradability of a self-isolated C. trogii TS01 on industrial lignins, including enzymatic hydrolysis lignin (EHL) and Kraft lignin (KL), was investigated in this paper. The results indicated that EHL could be used as an efficient carbon source to promote the cell growth and ligninolytic enzyme secretion of C. trogii TS01. Compared with using 2% glucose as carbon source, 1% EHL plus 1% glucose would increase the maximum cell dry weight, laccase activity, and manganese-dependent peroxidase activity of C. trogii TS01 by 24.8%, 164.1%, and 200%, respectively. However, the cell growth and ligninolytic enzyme secretion would be significantly inhibited in the case of 1% KL plus 1% glucose used as carbon source. As a result, at the 12th day of fermentation, the degradation rates of EHL and KL were 50.6% and 5.7%, respectively. The UV and FTIR analysis indicated that after been fermented by C. trogii TS01, S-unit content in EHL was decreased by 12.5% but G-unit content was increased by 53.7%. In conclusion, the research of this paper will provide a promising solution for the valorization of enzymatic hydrolysis lignin since the high biodegradation rate of lignin and high activity of ligninolytic enzymes could be achieved simultaneously.

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Abbreviations

EHL:

Enzymatic hydrolysis lignin

KL:

Kraft lignin

LiP:

Lignin peroxidase

MnP:

Mn-dependent peroxidase

Lac:

Laccase

PDA:

Potato dextrose agar

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

SLC:

Soluble lignin compounds

HMF:

5-Hydroxymethylfurfural

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Funding

This work was supported by the Specific Projects on Basic Research Cooperation of Beijing-Tianjin-Hebei (no. B2021204034); the Beijing Natural Science Foundation (no. J210013); and the Dalian National Laboratory for Clean Energy Cooperation Fund, CAS (no. DNL180305).

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  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, 1 North 2nd Street, Zhongguancun, Haidian District, Beijing, 100190, People’s Republic of China

    Weihua Qiu & Jinru Liu

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  1. Weihua Qiu
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  2. Jinru Liu
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All authors contributed to the study conception and design. Qiu Weihua: conceptualization, methodology, data curation, writing—original draft, writing—review and editing, supervision, project administration, funding acquisition; Jinru Liu: methodology, investigation, formal analysis, data curation, writing—original draft.

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Qiu, W., Liu, J. Fermenting and Lignin Degradability of a White-Rot Fungus Coriolopsis trogii Using Industrial Lignin as Substrate. Appl Biochem Biotechnol 194, 5220–5235 (2022). https://doi.org/10.1007/s12010-022-04004-5

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