Abstract
Species of Clavaria (Clavariaceae, Agaricales) collected from China were studied using morphological and molecular methods. Two species, C. aspersa and C. hupingshanensis, are here described as new to science; C. aspersa possesses simple, white basidiomata in gregarious or scattered fascicles, whereas C. hupingshanensis possesses simple, rose-white to seashell-pink basidiomata in gregarious to caespitose clusters. In addition, C. amoenoides is described as a newly recorded species for China; this species is characterized by simple, very pale orange-yellow to picric-yellow basidiomata. A phylogenetic analysis was conducted based on a combined dataset of internal transcribed spacer, nuclear ribosomal RNA large subunit, and the RNA polymerase II second largest subunit sequences. The phylogenetic reconstruction resolved accessions of the three species into three independent lineages within the Clavaria. The morphology of the three species is described in detail and is illustrated with line drawings and photographs. Holotypes of the new species are deposited in the Mycological Herbarium of Hunan Normal University. The sequences newly generated in this study have been deposited in GenBank. An updated key to the known Clavaria species in China is provided.
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Data availability
The sequence data generated in this study are deposited in NCBI GenBank.
References
Agnello C, Kautmanová I, Carbone M (2014) Clavaria messapica, una nuova specie del sud della Puglia. Rivista di Micologia 3:195–210
Agnello C, Papetti C (2020) Clavaria apulica una nuova specie dell’areale mediterraneo. Rivista di Micologia 63(2):121–130
Birkebak JM, Adamčík S, Looney BP, Matheny PB (2016) Multilocus phylogenetic reconstruction of the Clavariaceae (Agaricales) reveals polyphyly of agaricoid members. Mycologia 108:860–868. https://doi.org/10.3852/15-370
Birkebak JM, Mayor JR, Ryberg KM, Matheny PB (2013) A systematic, morphological and ecological overview of the Clavariaceae (Agaricales). Mycologia 105:896–911. https://doi.org/10.3852/12-070
Burt EA (1922) The North American species of Clavaria with illustrations of the type specimens. Ann Mo Bot Gard 9(1):1–78. https://doi.org/10.2307/2989963
Chen ZH, Zhang P (2019) Atlas of Macrofungi in Hunan. Hunan Normal University Press, Changsha
Chevallier FF (1826) Flore generale des environs de Paris: selon la methode naturelle. Ferra Jeune, Libraire, Paris
Coker WC (1923) The Clavarias of the United States and Canada. Dower publications, New York
Corner EJH (1950) A monograph of Clavaria and allied genera. Oxford University press, London
Corner EJH (1970) Supplement to “A monograph of Clavaria and allied genera”. Beihefte zur Nova Hedwigia 33:1–299
Corner EJH, Thind KS, Anand GPS (1956) The Clavariaceae of the Mussoorie Hills (India). II. Trans Br Mycol Soc 39(4):475–484. https://doi.org/10.1016/S0007-1536(57)80052-7
Cotton AD (1910) British clavariae. a correction. Trans Br Mycol Soc 3:265–266
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Edgar RC (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinf 5:113. https://doi.org/10.1186/1471-2105-5-113
Franchi P, Marchetti M (2021) I Funghi Clavarioidi in Italia. A. M. B, Vicenza
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Karich A, Kellner H, Schmidt M, Ullrich R (2015) A remarkable mycotope in the Zittau Mountains with the first record of Microglossum rufescens in Germany. Boletus 36(2):67–79
Kautmanová I, Tomšovský M, Dueñas M, Martín MP (2012) European species of Clavaria (Agaricales, Agaricomycetes) with dark basidiomata – a morphological and molecular study. Pers – Mol Phylogeny Evol Fungi 29:133–145. https://doi.org/10.3767/003158512x661543
Kornerup A, Wanscher JH (1978) Methuen handbook of colour, 3rd edn. Methuen, London
Linnaeus (1753) C. Species Plantarum. Impensis Laurentii Salvii, Stockholm
Liu LN, Wu L, Chen ZH, Bau T, Zhang P (2017) The species of Lentaria (Gomphales, Basidiomycota) from China based on morphological and molecular evidence. Mycol Prog 16:605–612. https://doi.org/10.1007/s11557-017-1284-2
Liu YJ, Whelen S, Hall BDI (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Mol Biol Evol 16(12):1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
Matheny PB, Wang Z, Binder M, Curtis JM, Hibbett DS (2007) Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Mol Phylogenet Evol 43(2):430–451. https://doi.org/10.1016/j.ympev.2006.08.024
Olariaga I, Huhtinen S, Læssøe T, Petersen JH, Hansen K (2020) Phylogenetic origins and family classification of typhuloid fungi, with emphasis on Ceratellopsis, Macrotyphula and Typhula (Basidiomycota). Stud Mycol 96:155–184. https://doi.org/10.1016/j.simyco.2020.05.003
Olariaga I, Salcedo I, Daniëls PP, Spooner B, Kautmanová I (2015) Taxonomy and phylogeny of yellow Clavaria species with clamped basidia—Clavaria flavostellifera sp. nov. and the typification of C. argillacea, C. flavipes and C. sphagnicola. Mycologia 107:104–122. https://doi.org/10.3852/13-315
Petersen RH (1978) The Genus Clavaria in South-eastern Australia. Aust J Bot 26:415–424
Petersen RH (1988) The clavarioid fungi of New Zealand. DSIR Science Information Publishing, Wellington
Rambaut A (2012) Figtree version 1.4.0. http://tree.bio.ed.ac.uk/software/figtree/
Ridgway R (1912) Color standards and color nomenclature. Published by the author, Washington
Roberts P (2007) Black & brown Clavaria species: in the British Isles. Field Mycology 8(2):59–62. https://doi.org/10.1016/s1468-1641(10)60454-9
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. https://doi.org/10.1093/bioinformatics/btg180
Stamatakis A (2006) RAxML-VI-HPC: maximum likelihoodbased phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690. https://doi.org/10.1093/bioinformatics/btl446
Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the raxml web servers. Syst Biol 57:758. https://doi.org/10.1080/10635150802429642
Stamatakis A, Ludwig T, Meier H (2005) RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. Bioinformatics 21:456–463. https://doi.org/10.1093/bioinformatics/bti191
Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods) Version 4. Sinauer Associates, Sunderland
Tai FL (1979) Sylloge Fungorum Sinicorum. Science Press, Beijing
Vaillant S (1727) Botanicon Parisiense, ou Dénombrement par Ordre Alphabétique des Plantes, qui se Trouvent dans les Environs de Paris. J. & H. Verbeek, Leiden, Amsterdam
Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies. In: PCR protocols, a guide to methods and applications. Academic, San Diego. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Wu CL, He Y, Yan J, Zhang P (2019) Two new species of Clavulina (Cantharellales) from southwestern China based on morphological and molecular evidence. Mycol Prog 18:1071–1078. https://doi.org/10.1007/s11557-019-01506-1
Yan J, Wang XY, Wang XH, Chen ZH, Zhang P (2020) Two new species of Clavaria (Agaricales, Basidiomycota) from Central China. Phytotaxa 477(1):71–80. https://doi.org/10.11646/phytotaxa.477.1.5
Acknowledgements
We thank Robert McKenzie, PhD, from Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript.
Funding
This study was financially supported by the National Natural Science Foundation of China (No. 31670015; No. 31750001, QYZDY-SSW-SMC029).
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Conceptualization: Ping Zhang; methodology: Jun Yan and Gui-Wu Li; performing the experiment: Jun Yan, Gui-Wu Li, and Wen-Hao Liu; resources: Ping Zhang, Zuo-Hong Chen, Gui-Wu Li, and Wen-Hao Liu; writing—original draft preparation: Jun Yan; writing—review and editing: Ping Zhang; supervision: Ping Zhang; project administration: Ping Zhang; funding acquisition: Ping Zhang. All authors have read and agreed to the published version of the manuscript.
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Yan, J., Li, GW., Liu, WH. et al. Updated taxonomy of Chinese Clavaria subg. Syncoryne (Clavariaceae, Agaricales): description of two new species and one newly recorded species. Mycol Progress 21, 67 (2022). https://doi.org/10.1007/s11557-022-01815-y
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DOI: https://doi.org/10.1007/s11557-022-01815-y