Abstract
Three new Phlebia species, Ph. fuscotuberculata, Ph. tomentopileata and Ph. tongxiniana, are proposed based on a combination of morphological features and molecular evidence. Phlebia fuscotuberculata is characterized by brown, tuberculate hymenial surface, fusiform cystidia and ellipsoid basidiospores. Phlebia tomentopileata is distinguished by effused or semi-pileate basidiomata with tomentose upper pileus surface and merulioid hymenophore and allantoid basidiospores (3.5–4.5 × 1–1.4 μm). Phlebia tongxiniana differs in its effused basidiomata with smooth, buff to cinnamon-buff hymenophore, monomitic hyphal system with clamped hyphae and ellipsoid basidiospores. Sequences of ITS and 28S gene regions of the studied specimens were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and Bayesian inference methods. The phylogenetic analyses based on combined ITS+28S dataset showed that the three new Phlebia species fell into the phlebioid clade. Further study focused on a more representative set of Phlebia species based on ITS+28S sequences demonstrated that Ph. fuscotuberculata was grouped with Ph. hydnoidea and Ph. nantahaliensis, Ph. tomentopileata was closely related to Ph. tremellosa and Ph. tongxiniana was grouped with Ph. chrysocreas.
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The research was supported by the National Natural Science Foundation of China (Project No. 31700023), Yunnan Agricultural Foundation Projects (2017FG001-042) and the Science Foundation of Southwest Forestry University (Projects No. 111715 and QN201904).
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Huang, RX., Zhao, CL. Three new species of Phlebia (Polyporales, Basidiomycota) based on the evidence from morphology and DNA sequence data. Mycol Progress 19, 753–767 (2020). https://doi.org/10.1007/s11557-020-01591-7
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DOI: https://doi.org/10.1007/s11557-020-01591-7