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Graphiola fimbriata: the first species of Graphiolaceae (Exobasidiales, Basidiomycota) described only based on its yeast stage

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Abstract

The systematic position of three yeast strains isolated from a plant cell culture, a piece of termite nest, or as a foliar endophyte of Coffea arabica, respectively, is evaluated using morphological, physiological, and phylogenetical characteristics. In culture, all three isolates produced white, pale orange to pink colored colonies of cylindrical cells with monopolar budding and pseudohyphae. Standard phenotypic, biochemical, physiological characterization, and phylogenetic analyses of the combined 26S rRNA gene (D1/D2 domains) and ITS region sequences showed the conspecificity of these isolates and suggest their placement within the Exobasidiales (Ustilaginomycotina) as a sister lineage of the sampled and sequenced Graphiola species. Here, we describe this species as Graphiola fimbriata sp. nov. MycoBank MB 825077 (holotype: PC1T; ex-type cultures: IBRC-M 30158T = CBS 13945T = DSM 104832T). This is the first species described in the genus Graphiola for which only the asexual, saprobic developmental phase is known. The description of the genus Graphiola is therefore emended to allow species known only from a saprobic state.

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Acknowledgements

The authors are grateful to C. Nirma for isolating SNB-CN72 and to R. Constantino for identification of the host termite. Authors are grateful to Prof. M. Catherine Aime (Purdue University, USA) and Dr. Marizeth Groenewald (Westerdijk Fungal Biodiversity Institute, The Netherlands) for granting access to the strain IBL 03150.

Funding

This work has benefited from an “Investissement d’Avenir” grant managed by the Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-0025).

The authors gratefully acknowledge financial support from the Iranian Biological Resource Centre (IBRC), ACECR.

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Authors and Affiliations

  1. Microorganisms Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran

    Shaghayegh Nasr, Mohammad Ali Amoozegar & Seyed Abolhassan Shahzadeh Fazeli

  2. Plant Evolutionary Ecology, Institute of Evolution and Ecology, University of Tübingen, Auf der Morgenstelle 5, D-72076, Tübingen, Germany

    Matthias Lutz

  3. Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

    Mohammad Ali Amoozegar

  4. French National Centre for Scientific Research, Institut de Chimie des Substances Naturelles, UPR 2301, Université Paris-Saclay, 1 avenue de la Terrasse, 91198, Gif-sur-Yvette, France

    Véronique Eparvier & Didier Stien

  5. CNRS, Laboratoire de Biodiversité et Biotechnologie Microbienne, USR3579, Observatoire Océanologique, Sorbonne Université, 66650, Banyuls-sur-Mer, France

    Didier Stien

  6. Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran

    Seyed Abolhassan Shahzadeh Fazeli

  7. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Brunswick, Germany

    Andrey Yurkov

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  1. Shaghayegh Nasr
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Nasr, S., Lutz, M., Amoozegar, M.A. et al. Graphiola fimbriata: the first species of Graphiolaceae (Exobasidiales, Basidiomycota) described only based on its yeast stage. Mycol Progress 18, 359–368 (2019). https://doi.org/10.1007/s11557-018-1450-1

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