Abstract
Lepista sordida is an edible and medicinal mushroom, but, until now, it had to be collected from the wild. The present study is the first report of the successful cultivation of a wild strain of L. sordida from Thailand. The morphological description and molecular examination of the fungus are included, in order to confirm the identification of the species. Optimization was carried out for mycelium growth and fruiting body production. yeast malt extract (YMA), pH 6.0 - 7.0 and a temperature of 25 - 30 °C. A sorghum mushroom spawn was used for upscaling of the mycelium to be used for fruiting body production. Optimal conditions for the fruiting phase were 25 °C with 95–97% humidity in a compost rice straw medium with sandy-soil casing layer. Additionally, the secondary metabolites of fruiting body and cultured mycelium were investigated. Nudic acid B, a known toxic polyacetylene, was isolated from submerged cultures of L. sordida, while no polyacetylenic compounds were found in the fruiting bodies.
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Acknowledgements
Financial support by the German Academic Exchange Service (DAAD) and the Thai Royal Golden Ph.D. Jubilee-Industry program (RGJ) for a joint TRF-DAAD PPP academic exchange grant to B.T., K.D.H., and M.S., and the RGJ for a personal stipend no. Ph.D/0138/2553 in 4.S.MF/53/A.3 to B.T. is gratefully acknowledged. K.D.H. would like to thank the Thailand Research Fund for a grant (BRG5580009) to study the taxonomy, phylogeny, and biochemistry of Thai basidiomycetes. Sequences from the University of Wyoming were provided with funding from NSF grant DEB-1050292 to S.L.M. The help of Anan Thawthong and Kritsana Jatuwong at Mae Fah Luang University in carrying out mushrooms harvesting is acknowledged.
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Section Editor: Zhu-Liang Yang
This article is part of the “Special Issue in honour of the 70th birthday of Dr. Eric McKenzie”.
Benjarong Thongbai and Christian Richter contributed equally to this work.
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Thongbai, B., Wittstein, K., Richter, C. et al. Successful cultivation of a valuable wild strain of Lepista sordida from Thailand. Mycol Progress 16, 311–323 (2017). https://doi.org/10.1007/s11557-016-1262-0
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DOI: https://doi.org/10.1007/s11557-016-1262-0