Abstract
The ontogeny of the myxomycete Physarum globuliferum was observed on corn meal agar and hanging drop cultures without adding sterile oat flakes, bacteria or other microorganisms. Its complete life cycle including spore germination, myxamoebae, swarm cells, plasmodial development, and maturity of fructifications was demonstrated. Details of spore-to-spore development are described and illustrated.
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References
Alexopoulos CJ (1969) The experimental approach to the taxonomy of the myxomycetes. Mycologia 61:219–239
Alexopoulos CJ, Mims CW, Blackwell M (1996) Introductory mycology, 4th edn. Wiley, New York
Ashworth JM, Jennifer D (1975) The biology of slime moulds. Edward Arnold Publishers, Limited, London, pp 1–31
Clark J (1995) Myxomycete reproductive systems: additional information. Mycologia 87:779–786
Clark J, Stephenson SL (1994) Didymium ovoideum culture and mating system. Mycologia 86:392–396
Clark J, Brown D, Hu FS (1990) Growth of the myxomycete Stemonitis flavogenita on a defined minimal medium. Mycologia 82:385–386
Collins OR (1979) Myxomycete biosystematics: some recent developments and future research opportunities. Bot Rev 45:145–201
Gray WD (1938) The effect of light on the fruiting of myxomycetes. Amer J Bot 25:511–522
Gray WD, Alexopoulos CJ (1968) Biology of myxomycetes. The Ronald Press Company, New York
Gustafson RA, Thurston EL (1974) Calcium deposition in the myxomycete Didymium squamulosum. Mycologia 66:397–412
Haskins EF (1988) Miss Gulielma Lister’s observations on the myxomycete Echinostelium minutum. Mycologia 80:853–855
Haskins EF, Wrigley de Basanta D (2008) Methods of agar culture of myxomycetes: an overview. Revista Mexicana de Micologia 27:1–7
He J, Zhang XX, Gao Y, Li SJ, Sun YQ (2008) Effects of altered gravity on the cell cycle, actin cytoskeleton and proteome in Physarum polycephalum. Acta Astronaut 63:915–922
Henney MR (1968) Mating type systems in myxomycetes Physarum globuliferum and Physarum bilgramii. Amer J Bot 55:720
Henney HR, Lynch T (1969) Growth of Physarum flavicomum and Physarum rigidum in chemically defined minimal media. J Bact 99:531–534
Henney MR, Alexopoulos CJ, Scheetz RW (1980) Didymium atrichum, a new myxomycete from south-central Texas. Mycotaxon 11:150–164
Hok KA (1954) Study of the nutrition of myxomycete plasmodia. Amer J Bot 41:792–799
Hosoda E (1980) Culture methods and sporulation of Physarum polycephalum. Mycologia 72:500–504
Hosoda E (1981) Sporulation of oat-cultured Physarum polycephalum. I. Sporulation competence of dark starved plasmodia. Mycologia 73:689–695
Hosoda E (1983) Sporulation of oat-cultured Physarum polycephalum. II. Sporulation competence of slow desiccation-induced sclerotia. Mycologia 75:14–21
Howard FL (1931) The life history of Physarum polycephalum. Amer J Bot 18:116–133
Hu FS, Clark J (1986) Axenic culture of the myxomycete Stemonitis flavogenita. Mycologia 78:478–482
Ishibashi M, Iwasaki T, Imai S, Sakamoto S, Yamaguchi K, Ito A (2001) Laboratory culture of the myxomycetes, formation of fruiting bodies of Didymium bahiense and its plasmodial production of Makaluvamine A. J Nat Prod 64:108–110
Jampens DT, Bailey J, Cook LJ, Constantin B, Vandekerckhove J, Gettemans J (1999) Physarum amoebae express a distinct fragmin-like actin-binding protein that controls in vitro phosphorylation of actin by the actin-fragmin kinase. Eur J Biochem 265:240–250
Karl M, Anderson R, Holler E (2004) Injection of poly(β-L-malate) into the plasmodium of Physarum polycephalum shortens the cell cycle and increases the growth rate. Eur J Biochem 271:3805–3811
Keller HW, Braun KL (1999) Myxomycetes of Ohio: their systematics, biology and use in teaching. Ohio Biol Surv 13(2):1
Keller HW, Schoknecht JD (1989a) Life cycle of a new annulate-spored species of Didymium. Mycologia 81:248–265
Keller HW, Schoknecht JD (1989b) Spore-to-spore culture of Physarum spinisporum and its transfer to Badhamia. Mycologia 81:631–636
Keller HW, Schoknecht JD (1989c) Spore-to-spore cultivation of a new wrinkled- reticulate-spored Badhamia. Mycologia 81:783–789
Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Dictionary of the fungi, 10th edn. Cromwell Press, Trowbridge, pp 765–766
Koevenig JL (1964) Studies on life cycle of Physarum gyrosum and other myxomycetes. Mycologia 56:170–184
Lado C, Mosquera J, Estrada-Torres A, Beltrán-Tejera E, Wrigley de Basanta D (2007) Description and culture of a new succulenticolous Didymium (Myxomycetes). Mycologia 99:602–611
Lister A (1901) On the cultivation of mycetoza from spores. J Bot 39:57–63
Martin GW, Alexopoulos CJ (1969) The myxomycetes. University of Iowa Press, Iowa, p 279
Martin GW, Alexopoulos CJ, Farr ML (1983) The genera of myxomycetes. University of Iowa Press, Iowa, p 62
McManus SMA (1964) The plasmodia of the myxomycetes Licea biforis and Cribraria violacea. Mycologia 56:237–239
Mosquera J, Lado C, Estrada-Torres A, Beltrán-Tejera E, Wrigley de Basanta D (2003) Description and culture of a new myxomycete, Licea succulenticola. Anales Jardín Botánico de Madrid 60:1–10
Nannenga-Bremekamp NE (1991) A guide to temperate myxomycetes (trans: Feest A, Burggraaf Y). Biopress Limited, pp 198–199
Ross IK (1967) Growth and development of the myxomycete Perichaena vermicularis. I. Cultivation and vegetative nuclear divisions. Amer J Bot 54(5):617–625
Shimada Y, Nakano M, Kanda N, Murakami-Murofushi K, Kim JK, Ide T, Murofushi H (1997) Cell cycle-dependent activation of telomerase in naturally synchronized culture of a true slime mold, Physarum polycephalum. Biochem Biophys Res Commun 232:492–496
Stephenson SL, Stempen H (1994) Myxomycetes: a handbook of slime molds. Timber press, Inc, New York, pp 145–146
Wang Q, Nian SQ, Li Y (2006) Comparative study on ontogeny of Trichiales (Myxomycota) (in Chinese). Mycosystema 25:31–40
Wrigley de Basanta D, Lado C, Estrada-Torres A (2008) Morphology and life cycle of a new species of Didymium (Myxomycetes) from arid areas of Mexico. Mycologia 100(6):921–929
Zhu H, Wang Q (2004) Preliminary study on ontogeny of Trichiales myxomycetes by agar culture (in Chinese). J Yunnan Agric Univ 19:365–367
Acknowledgments
We thank Profs. Harold W. Keller, John Webster, Ian R. Hall, and Tom Hsiang for their valuable revisions and kind help. We also thank staffs of the Archives of Microbiology, chief editor Erko Stackebrandt and editorial assistant Susanne Verbarg for their patient revisions and suggestions for this paper. This study was supported by the National Natural Science Foundation of China (Project Nos. 30270008, 30670015).
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Communicated by Erko Stackebrandt.
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Liu, P., Wang, Q. & Li, Y. Spore-to-spore agar culture of the myxomycete Physarum globuliferum . Arch Microbiol 192, 97–101 (2010). https://doi.org/10.1007/s00203-009-0533-4
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DOI: https://doi.org/10.1007/s00203-009-0533-4