Table 1.
The measured features and their symbols.
Table 2.
Primers used for amplification and sequencing with PCR reaction conditions.
Table 3.
Voucher information and accession numbers for the specimens included in the phylogenetic analysis of chloroplast markers.
Fig 1.
PCA of the leaf cell width and length measurements of all tested P. nemorale sensu lato specimens.
Green triangles–group of specimens of P. longisetum, white triangles–molecularly examined specimens of P. longisetum (Wolski1, Wolski5, Wolski12, Wolski14-15, Wolski17, Wolski19, Wolski22-26, Wolski28-29), black triangle–P. longisetum type, red circles–group of specimens of P. nemorale sensu stricto, black circle–P. nemorale type.
Fig 2.
Gametophyte and sporophyte as well as stem leaves and cells from individual zones of the P. longisetum.
Drawn from the type material (PC132572, H-SOL 1563011) G. J. Wolski & A. Cienkowska.
Fig 3.
PCA of the leaf cell width and length measurements of P. longisetum and P. angusticellum specimens.
Green squares–P. longisetum, white squares–molecularly examined specimens of P. longisetum (Wolski12, Wolski14, Wolski15, Wolski17, Wolski19), black square–P. longisetum type, green rhombus–P. angusticellum, white rhombus–molecularly examined specimens of P. angusticellum (Wolski1, Wolski5, Wolski22-23, Wolski24-26, Wolski28-29).
Fig 4.
Phylogenetic tree based on ITS sequences (total 710 bp) showing the position of P. longisetum specimens among other Plagiothecium and similar hypnalean mosses.
Numbers on branches indicate bootstrap values from ML followed by posterior probabilities from BI analysis. Asterisk (*) indicates 100 (ML) and 1.00 (BI), while minus (-) indicates values below 50 (ML, BI). The topology of the tree was based on ML analysis.
Fig 5.
Phylogenetic tree of Plagiothecium group with Pseudotaxiphyllum as the outgroup taxa based on concatenated nuclear (ITS) and chloroplast (rps4 and rpl16) DNA markers (total 2068 bp).
The tree shows the position of P. longisetum specimens among the Plagiothecium group. Numbers on branches indicate bootstrap values from ML followed by posterior probabilities from BI analysis. Asterisk (*) indicates 100 (ML. MP) and 1.00 (BI), while minus (-) indicates values below 80 (ML) and 75 (BI). The topology of the tree was based on ML analysis.
Fig 6.
Gametophyte, stem leaves and cells from individual leaf zones of the P. angusticellum.
Drawn from the holotype (LOD 14927) G. J. Wolski & A. Cienkowska.
Table 4.
Comparison of the main diagnostic features of the described species.