Objective: Urbanization has been reported to affect the biodiversity of myxomycetes. There is no data in the Bursa city on the diversity of myxomycete in the urban area. Therefore, we aim to determine the presence of corticolous myxomycetes on monumental trees that are located in the most populated province of Bursa city (Osmangazi and Yildirim). Materials and Methods: In August and October 2018, 100 barks of monumental trees located in the Osmangazi and Yildirim) county of Bursa was collected. Moist chamber culture technique was used for the isolation and identification of the species. Results: 16 species within 9 genera were identified. The species identified are listed, and six new species Didymium bahiense (Gottsberger), Didymium difforme (Pers.) S. F. Gray), Macbrideola martinii (Alexop. & Beneke), Macbrideola oblonga (Pando, Lado), Physarum gyrosum (Rost.), Physarum notabile (Macbr.) were recorded for Bursa city. Conclusion: This is the first report of corticolous myxomycetes on monumental trees in Bursa city center. It also added data and 6 new records of myxomycetes on the myxomycete biodiversity of Bursa. Keywords: Bursa, corticolous, moist chamber, monumental, myxomycetes, turkey